General Information
-
DRAMP ID
- DRAMP21460
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Peptide Name
- PQ (De Novo Synthesis)
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Source
- synthetic construct
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Family
- Not found
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Gene
- Not found
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Sequence
- IHKFWRCRRRFCRWFKHI
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Sequence Length
- 18
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.30538681] Gram-positive bacteria:Staphylococcus aureus ATCC 29213(MIC=8μM), Staphylococcus aureus ATCC 43300(MIC=8μM), Staphylococcus epidermidis ATCC 12228(MIC=8μM), Streptococci faecalis ATCC 29212(MIC=16μM), Bacillus subtilis CMCC 63501(MIC=16μM);
- Gram-negative bacteria:Escherichia coli ATCC 25922(MIC=16μM), Escherichia coli UB 1005(MIC=8μM), bacteria pyocyaneum ATCC 27853(MIC=8μM), Salmonella typhimurium ATCC 7731(MIC=8μM), Salmonella Pulloru C79-13(MIC=8μM)
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Hemolytic Activity
-
- [Ref.30538681] MHC>128μM against human blood cells.The minimum hemolytic concentration (MHC) is defined as the peptide concentration resulting in 5% hemolysis.
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Cytotoxicity
-
- [Ref.30538681] The cell viability of intestinal epithelial cells (IPEC-J2) induced by PQ is 68.2%, 68.6%, 66.8%,63.6%, 69.1%, 62.2%, 55.8% and 28.1% at peptide concentrations of 1, 2, 4, 8, 16, 32, 64 and 128 μM.
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
-
Structure
- α-helix predicted by I-Tasser
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Structure Description
- In PBS, the α-helix content of PQ is 26.91% and the β-strand content of that is 11.16%. In SDS, the α-helix content of PQ is 32.02% and the β-strand of that is 6.15%. In TFE, the α-helix content of PQ is 36.82% and the β-strand of that is 4.28%.
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21460.
Physicochemical Information
-
Formula
- C121H179N41O19S2
Absent Amino Acids
- ADEGLMNPQSTVY
Common Amino Acids
- R
Mass
- 2576.14
PI
- 11.84
Basic Residues
- 9
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +9
-
Boman Index
- -6902
Hydrophobicity
- -0.894
Aliphatic Index
- 43.33
Half Life
-
- Mammalian:20 hour
- Yeast:30 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 11125
Absorbance 280nm
- 654.41
Polar Residues
- 2
DRAMP21460
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.
Literature Information
- ·Literature 1
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Title
- Short Symmetric-End Antimicrobial Peptides Centered on β-Turn Amino Acids Unit Improve Selectivity and Stability
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Pubmed ID
- 30538681
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Reference
- Front Microbiol. 2018; 9: 2832. Published online 2018 Nov 27. doi: 10.3389/fmicb.2018.02832
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Author
- Na Dong, Shuli Chou, Jiawei Li, Chenyu Xue, Xinran Li, Baojing Cheng, Anshan Shan, and Li Xu