General Information
-
DRAMP ID
- DRAMP21465
-
Peptide Name
- P1-Ll-1577 (De Novo Synthesis)
-
Source
- Leptodactylus latrans (Anura: Leptodactylidae)
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- DEMKLDGFNMHLE
-
Sequence Length
- 13
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.30423858] Gram-positive bacteria:Staphylococcus aureus ATCC 25923(MIC=40.5μM);
- Gram-negative bacteria:Escherichia coli ATCC 25922(MIC=20μM)
-
Hemolytic Activity
-
- [Ref.30423858] 100% hemolysis MHC=640μM against human blood cells
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Random coil predicted by PSIPRED and GOR V
-
Structure Description
- P1-Ll-1577, although presenting contributions of turn structure, is less ordered in the presence of TFE (percentage of an unordered structure higher than 40%).
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21465.
Physicochemical Information
-
Formula
- C67H103N17O23S2
Absent Amino Acids
- ACIPQRSTVWY
Common Amino Acids
- DELM
Mass
- 1578.77
PI
- 4.31
Basic Residues
- 2
Acidic Residues
- 4
Hydrophobic Residues
- 3
Net Charge
- -2
-
Boman Index
- -2945
Hydrophobicity
- -0.831
Aliphatic Index
- 60
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 2
DRAMP21465
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria and Gram-nagetive bacteria.
Literature Information
- ·Literature 1
-
Title
- Leptodactylus latrans Amphibian Skin Secretions as a Novel Source for the Isolation of Antibacterial Peptides
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Pubmed ID
- 30423858
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Reference
- Molecules. 2018 Nov; 23(11): 2943. Published online 2018 Nov 11. doi: 10.3390/molecules23112943
-
Author
- Alvaro Siano, Maria Veronica Humpola, Eliandre de Oliveira, Fernando Albericio, Arturo C Simonetta, Rafael Lajmanovich, Georgina G Tonarelli