General Information
-
DRAMP ID
- DRAMP21626
-
Peptide Name
- E2EM23W
-
Source
- Synthetic construct
-
Family
- Not found
-
Gene
- Not found
-
Sequence
- GILDTLKQFAKGVGKWLVKGAAQ
-
Sequence Length
- 23
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
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Target Organism
-
- [Ref.24211019] Gram-positive bacteria: Bacillus subtilis (MIC = 50 μg/mL), Staphylococcus aureus (MIC = 100 μg/mL), Staphylococcus epidermis (MIC > 200 μg/mL);
- Gram-negative bacteria: Escherichia coli (MIC > 200 μg/mL), Shigella dysentariae (MIC > 200 μg/mL), Salmonella typhimurium (MIC > 200 μg/mL), Klebsiella pneumonia (MIC > 200 μg/mL), Proteus mirabilis (MIC > 200 μg/mL), Pseudomonas aeuginose (MIC > 200 μg/mL).
-
Hemolytic Activity
-
- [Ref.24211019] No hemolytic activity information found.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Acetylation
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Typical random coil conformation with only 14% helical content in a 25 mM potassium phosphate buffer solution at 20 ℃.
-
Structure Description
- E2EM23W showed circular dichroism spectra that are typical for a random coil, and its helical content was only 14%.
-
Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP21626.
Physicochemical Information
-
Formula
- C₁₁₃H₁₈₆N₃₀O₂₉
Absent Amino Acids
- CEHMNPRSY
Common Amino Acids
- GK
Mass
- 2428.9
PI
- 10
Basic Residues
- 4
Acidic Residues
- 1
Hydrophobic Residues
- 11
Net Charge
- +3
-
Boman Index
- -231
Hydrophobicity
- 0.139
Aliphatic Index
- 106.09
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 250
Polar Residues
- 5
DRAMP21626
Comments Information
Function
- Antibacterial activity against Gram-positive bacteria. Antibacterial activity against Gram-negative bacteria is not noteable under 200 μg/mL.
Literature Information
- ·Literature 1
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Title
- Truncated and constrained helical analogs of antimicrobial esculentin-2EM
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Pubmed ID
- 24211019
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Reference
- Bioorg Med Chem Lett. 2013 Dec 15;23(24):6717-20. doi: 10.1016/j.bmcl.2013.10.031. Epub 2013 Oct 26.
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Author
- Thanh Kim Pham, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim