General Information

    • DRAMP ID

    • DRAMP21627

    • Peptide Name

    • E2EM15W

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • TLKQFAKGVGKWLVK

    • Sequence Length

    • 15

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.24211019] Gram-positive bacteria: Bacillus subtilis (MIC = 200 μg/mL), Staphylococcus aureus (MIC = 100 μg/mL), Staphylococcus epidermis (MIC > 200 μg/mL);
      • Gram-negative bacteria: Escherichia coli (MIC = 200 μg/mL), Shigella dysentariae (MIC > 200 μg/mL), Salmonella typhimurium (MIC > 200 μg/mL), Klebsiella pneumonia (MIC > 200 μg/mL), Proteus mirabilis (MIC > 200 μg/mL), Pseudomonas aeuginose (MIC > 200 μg/mL).

    • Hemolytic Activity

      • [Ref.24211019] No hemolytic activity information found.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Acetylation

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Random coil coformation with 4% helical content in a 25 mM potassium phosphate buffer solution at 20 ℃.

    • Structure Description

    • Circular dichroism also revealed that E2EM15W is even less structured under the same conditions, displaying a helical content of 4%; it is well established that the shorter a peptide is, the more difficult it is to form an α-helical conformation.

    • Helical Wheel Diagram

    • DRAMP21627 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21627.

Physicochemical Information

    • Formula

    • C₈₂H₁₃₅N₂₁O₁₈

    • Absent Amino Acids

    • CDEHIMNPRSY

    • Common Amino Acids

    • K

    • Mass

    • 1703.1

    • PI

    • 10.48

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +4

    • Boman Index

    • -339

    • Hydrophobicity

    • -0.06

    • Aliphatic Index

    • 97.33

    • Half Life

      • Mammalian:7.2 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 392.86

    • Polar Residues

    • 3

DRAMP21627

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria. Antibacterial activity against Gram-negative bacteria is not noteable under 200 μg/mL.

Literature Information

  • ·Literature 1

    • Title

    • Truncated and constrained helical analogs of antimicrobial esculentin-2EM

    • Reference

    • Bioorg Med Chem Lett. 2013 Dec 15;23(24):6717-20. doi: 10.1016/j.bmcl.2013.10.031. Epub 2013 Oct 26.

    • Author

    • Thanh Kim Pham, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim