General Information
-
DRAMP ID
- DRAMP21637
-
Peptide Name
- SLAY-P7
-
Source
- Synthetic construct after SLAY screening and prediction
-
Family
- N/A
-
Gene
- N/A
-
Sequence
- IHRDQQHESFLDARPEPGLTE
-
Sequence Length
- 21
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
-
Target Organism
-
- [Ref.29307492] Gram-negative bacteria: E. coli W3110 (MBC = 4 μM), Acinetobacter baumannii 5075 (MBC = 16 μM), Pseudomonas aeruginosa 14 (MBC = 4 μM), E. coli conferring New Delhi metallo-beta-lactamase (MBC = 4 μM).
-
Hemolytic Activity
-
- [Ref.29307492] The peptide showed 0.7% hemolysis against human red blood cells at 50 μM. PBS was uesd as a negative control and 1% triton was used as a positive control for 100% lysis.
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Unknown
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Unknown
-
Structure Description
- Not mentioned
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21637.
Physicochemical Information
-
Formula
- C106H163N33O36
Absent Amino Acids
- CKMNVWY
Common Amino Acids
- E
Mass
- 2475.66
PI
- 4.87
Basic Residues
- 4
Acidic Residues
- 5
Hydrophobic Residues
- 5
Net Charge
- -1
-
Boman Index
- -7359
Hydrophobicity
- -1.348
Aliphatic Index
- 60.48
Half Life
-
- Mammalian:20 hour
- Yeast:30 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 3
DRAMP21637
Comments Information
Function
- Antibacterial activity against Gram-negative bacteria.
Literature Information
- ·Literature 1
-
Title
- Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries
-
Pubmed ID
- 29307492
-
Reference
- Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.
-
Author
- Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies