General Information
-
DRAMP ID
- DRAMP21644
-
Peptide Name
- SLAY-P14
-
Source
- Synthetic construct after SLAY screening and prediction
-
Family
- N/A
-
Gene
- N/A
-
Sequence
- SMLAYVDKNDHINPPHSPRS
-
Sequence Length
- 20
-
UniProt Entry
- No entry found
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
-
Target Organism
-
- [Ref.29307492] Gram-negative bacteria: E. coli W3110 (MBC = 32 μM), Acinetobacter baumannii 5075 (MBC = 128 μM), Pseudomonas aeruginosa 14 (MBC = 32 μM), E. coli conferring New Delhi metallo-beta-lactamase (MBC = 64 μM).
-
Hemolytic Activity
-
- [Ref.29307492] The peptide showed 1.5% hemolysis against human red blood cells at 50 μM. PBS was uesd as a negative control and 1% triton was used as a positive control for 100% lysis.
-
Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
-
Binding Target
- Unknown
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Free
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Unknown
-
Structure Description
- Not mentioned
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP21644.
Physicochemical Information
-
Formula
- C98H152N30O31S
Absent Amino Acids
- CEFGQTW
Common Amino Acids
- PS
Mass
- 2278.53
PI
- 6.66
Basic Residues
- 4
Acidic Residues
- 2
Hydrophobic Residues
- 4
Net Charge
- +2
-
Boman Index
- -5281
Hydrophobicity
- -1.055
Aliphatic Index
- 58.5
Half Life
-
- Mammalian:1.9 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 1490
Absorbance 280nm
- 78.42
Polar Residues
- 6
DRAMP21644
Comments Information
Function
- Antibacterial activity against Gram-negative bacteria.
Literature Information
- ·Literature 1
-
Title
- Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries
-
Pubmed ID
- 29307492
-
Reference
- Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.
-
Author
- Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies