General Information

    • DRAMP ID

    • DRAMP21648

    • Peptide Name

    • SLAY-P18

    • Source

    • Synthetic construct after SLAY screening and prediction

    • Family

    • N/A

    • Gene

    • N/A

    • Sequence

    • CNPLNGADRRTDSFPRFTVI

    • Sequence Length

    • 20

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.29307492] Gram-negative bacteria: E. coli W3110 (MBC = 128 μM), Acinetobacter baumannii 5075 (MBC > 128 μM), Pseudomonas aeruginosa 14 (MBC = 64 μM), E. coli conferring New Delhi metallo-beta-lactamase (MBC > 128 μM).

    • Hemolytic Activity

      • [Ref.29307492] The peptide showed 1.7% hemolysis against human red blood cells at 50 μM. PBS was uesd as a negative control and 1% triton was used as a positive control for 100% lysis.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Unknown

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Unknown

    • Structure Description

    • Not mentioned

    • Helical Wheel Diagram

    • DRAMP21648 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP21648.

Physicochemical Information

    • Formula

    • C98H155N31O30S

    • Absent Amino Acids

    • EHKMQWY

    • Common Amino Acids

    • R

    • Mass

    • 2279.56

    • PI

    • 8.25

    • Basic Residues

    • 3

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +1

    • Boman Index

    • -6015

    • Hydrophobicity

    • -0.545

    • Aliphatic Index

    • 58.5

    • Half Life

      • Mammalian:1.2 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 7

DRAMP21648

DRAMP21648 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-negative bacteria.

Literature Information

  • ·Literature 1

    • Title

    • Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries

    • Reference

    • Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.

    • Author

    • Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies