General Information

    • DRAMP ID

    • DRAMP29005

    • Peptide Name

    • Unstapled heptapeptide 9

    • Source

    • Synthetic construct

    • Family

    • N/A

    • Gene

    • N/A

    • Sequence

    • KLLKLLK

    • Sequence Length

    • 7

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC = 49 ± 11 μg/mL). Methicillin-resistant Staphylococcus aureus (MRSA) (MIC = 41 ± 9 μg/mL)

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Helicity = 33.7% in 10 mM sodium phosphate buffer (pH 7.4) at peptide concentrations of 100 μM

    • Structure Description

    • CD spectroscopy was used to characterize the secondary structure of five unstapled heptapeptides and their stapled counterparts in phosphate buffer, indicating a significant increase in peptide helical content upon the stapling, with helicity change from h = 14.1% - 33.7% (for unstapled peptides) to h = 58.9%-75.1% (for stapled peptides).

    • Helical Wheel Diagram

    • DRAMP29005 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29005.

Physicochemical Information

    • Formula

    • C42H82N10O8

    • Absent Amino Acids

    • ACDEFGHIMNPQRSTVW

    • Common Amino Acids

    • L

    • Mass

    • 855.18

    • PI

    • 10.3

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 4

    • Net Charge

    • +3

    • Boman Index

    • 303

    • Hydrophobicity

    • 0.5

    • Aliphatic Index

    • 222.86

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 0

DRAMP29005

Comments Information

    • Function

    • Antibacterial activity against Gram-positive bacteria.

    • It is a heptapeptide designed rationally.

Literature Information

  • ·Literature 1

    • Title

    • De Novo Hydrocarbon-Stapling Design of Single-Turn α-Helical Antimicrobial Peptides

    • Reference

    • Int J Pept Res Ther. 2019 Nov 14; 26(4):1711–1719. doi: 10.1007/s10989-019-09964-7.

    • Author

    • Zhixia Chen, Xiuli Yu, Aiying Zhang, Fangfang Wang, Yankun Xing