General Information

    • DRAMP ID

    • DRAMP29006

    • Peptide Name

    • A4K14-citropin1.1

    • Source

    • Synthetic construct

    • Family

    • N/A

    • Gene

    • N/A

    • Sequence

    • GLFAVIKKVASVIKGL

    • Sequence Length

    • 16

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Anticancer

    • Target Organism

      • [Ref.33363118] Cancer cell lines: C4-2B (IC50 = 29.05 μM), A549 (IC50 = 14.97 μM), U87 (IC50 = 14.8 μM), MCF-7 (14.16 μM)

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Acetylation

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Helicity = 61.5% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)

    • Structure Description

    • [Ref.33363118] CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.

    • Helical Wheel Diagram

    • DRAMP29006 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29006.

Physicochemical Information

    • Formula

    • C79H139N19O18

    • Absent Amino Acids

    • CDEHMNPQRTWY

    • Common Amino Acids

    • KV

    • Mass

    • 1643.09

    • PI

    • 10.3

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 10

    • Net Charge

    • +3

    • Boman Index

    • 2023

    • Hydrophobicity

    • 1.394

    • Aliphatic Index

    • 164.38

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP29006

Comments Information

    • Function

    • Antitumor activity against A549, HCT116 and HepG2 cancer cells.

    • A4K14-citropin 1.1 is a structurally optimized derivative of citropin-1.1 derived from amphibians' skin secreta peptide Citropin, which exhibits broad biological activities. Citropin-1.1 is Amphibian defense peptide with antibiotic and antimicrobial activity. Bowie and his team found that replacement of Asp4 and Gly14 with Ala and Lys (termed A4K14-CITROPIN 1.1) resulted in a more stable α-helix than Citropin on the C-terminal section, and it led to better biologicla activities

Literature Information

  • ·Literature 1

    • Title

    • Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides

    • Reference

    • Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.

    • Author

    • Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu