General Information

    • DRAMP ID

    • DRAMP29031

    • Peptide Name

    • Stripe-based foldamer peptide 3

    • Source

    • Synthetic construct

    • Family

    • N/A

    • Gene

    • N/A

    • Sequence

    • KLLKKZGKLLKKZGKLLKKZG

    • Sequence Length

    • 21

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.33369262] Gram-positive bacteria: Staphylococcus aureus NBRC13276 (MIC = 6.25 μM) ;
      • Gram-negative bacteria: Escherichia coli DH5α (MIC = 3.125 μM), Pseudomonas aeruginosa NBRC13275 (MIC = 6.25 μM), multidrug-resistant Pseudomonas aeruginosa ATCCBAA-2111 (MDRP) (MIC = 12.5 μM)

    • Hemolytic Activity

      • [Ref.33369262] It exhibits hemolysis at 1.56 μM agasint human red blood cells.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • The Z (position: 6, 13 and 20) in sequence denote Ac₆c residue (1-Aminocyclohexanecarboxylic acid).

    • Stereochemistry

    • L

    • Structure

    • α-Helix content = 49% in 20 mM phosphate buffered saline (PBS) solution (pH 7.4), with 1% sodium dodecyl sulfate

    • Structure Description

    • [Ref.33369262] As shown in Figure 2, peptides 2, 3, 4 and 5 showed negative maxima at around 208 and 222nm, which suggests that they formed stable α-helical structures, similar to Stripe.

    • Helical Wheel Diagram

    • DRAMP29031 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29031.

Physicochemical Information

    • Formula

    • C96H179N27O16

    • Absent Amino Acids

    • ACDEFHIMNPQRSTVWY

    • Common Amino Acids

    • K

    • Mass

    • 2407.94

    • PI

    • 10.9

    • Basic Residues

    • 9

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Net Charge

    • +9

    • Boman Index

    • -1761

    • Hydrophobicity

    • -0.643

    • Aliphatic Index

    • 111.43

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP29031

DRAMP29031 chydropathy plot

Comments Information

    • Function

    • Antibacterial activity against Gram-negative bacteria.

    • It is a helical foldmer peptide based on "Stripe" (an AMP manually designed) by introducing hydrophobic α,α-disubstituted amino acids (1-aminocyclohexanecarboxylic acid Ac₆c). The peptide showed similar or silightly weaker activity than Stripe.

Literature Information

  • ·Literature 1

    • Title

    • Rational Design of Helix-Stabilized Antimicrobial Peptide Foldamers Containing α,α-Disubstituted Amino Acids or Side-Chain Stapling

    • Reference

    • Chempluschem. 2020 Dec;85(12):2731-2736. doi: 10.1002/cplu.202000749.

    • Author

    • Motoharu Hirano, Chihiro Saito, Chihiro Goto, Hidetomo Yokoo, Ryuji Kawano, Takashi Misawa, Yosuke Demizu