General Information
-
DRAMP ID
- DRAMP29038
-
Peptide Name
- [P5K,D9K]Hymenochirin-1B
-
Source
- Synthetic construct
-
Family
- N/A
-
Gene
- N/A
-
Sequence
- IKLSKETKKNLKKVLKGAIKGAIAVAKMV
-
Sequence Length
- 29
-
UniProt Entry
- Notfound
-
Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Anticancer
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Target Organism
-
- [Ref.24172540] Gram-positive bacteria: S. aureus ATCC 29213 (MIC = 6.25 μM), S. aureus ATCC 25923 (MIC = 3.1 μM), S.epidermidis RP62A (MIC = 1.6 μM), S.epidermidis RP62A/1 (MIC = 1.6 μM), C.albicans ATCC 90028 (MIC = 50 μM);
- Gram-negative bacteria: E.coli (MIC = 6.25 μM), A. baumannii (MIC = 3.1 μM), S. maltophilia (MIC = 3.1 μM), K. pneumoniae (MIC = 6.25 μM), P. aeruginosa (MIC = 12.5 μM), P.mirabilis (MIC > 100 μM).
- [Ref.30789695] Cancer cell lines: A549 (IC50 = 1.17 ± 0.23 μM), HCT116 (IC50 = 4.93 ± 0.51 μM), HepG2 (IC50 = 2.46 ± 0.32 μM).
-
Hemolytic Activity
-
- [Ref.24172540] LC₅₀ = 127 ± 9 μM against freshly prepared human erythrocytes.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
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Binding Target
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- α-helical (most likely)
-
Structure Description
- No structure indentification experiment and detailed structure description found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP29038.
Physicochemical Information
-
Formula
- C142H263N39O35S
Absent Amino Acids
- CDFHPQRWY
Common Amino Acids
- K
Mass
- 3108.95
PI
- 10.54
Basic Residues
- 9
Acidic Residues
- 1
Hydrophobic Residues
- 13
Net Charge
- +8
-
Boman Index
- -1626
Hydrophobicity
- 0.076
Aliphatic Index
- 124.48
Half Life
-
- Mammalian:20 hour
- Yeast:30 min
- E.coli:>10 hour
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 5
DRAMP29038
Comments Information
Function
- Antibacterial activity against Gram-positive and Gram-negative bacteria; Anticancer activity.
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays
Literature Information
- ·Literature 1
-
Title
- An analog of the host-defense peptide hymenochirin-1B with potent broad-spectrum activity against multidrug-resistant bacteria and immunomodulatory properties.
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Pubmed ID
- 24172540
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Reference
- Peptides. 2013 Dec;50:153-9. doi: 10.1016/j.peptides.2013.10.015. Epub 2013 Oct 27.
-
Author
- Milena Mechkarska, Manju Prajeep, Gordana D Radosavljevic, Ivan P Jovanovic, Amna Al Baloushi, Agnes Sonnevend, Miodrag L Lukic, J Michael Conlon.
- ·Literature 2
-
Title
- Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Nov
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Pubmed ID
- 30789695
-
Reference
- ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.
-
Author
- Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao.