• • DRAMP ID

  • DRAMP29043

• • Peptide Name

  • Oncocin (variant of Oncopeltus antibacterial peptide 4)

• • Source

  • Synthetic construct (Oncocin is a variant of the 2 kDa Oncopeltus antibacterial peptide 4, which was originally isolated from oncopeltus fasciatus (milkweed bug))

• • Family

  • Not found

• • Gene

  • N/A

• • Sequence

  • VDKPPYLPRPRPPRRIYNR

• • Sequence Length

  • 19

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram-

• • Target Organism

  • • [Ref.20565063] Gram-negative pathogens: P.aeruginosa DSM3227 (MIC = 4 μg/mL), P. aeruginosa Walter (MIC = 8 μg/mL), P. aeruginosa ATCC27853 (MIC = 4 μg/mL), P. aeruginosa PAO1 (MIC = 2 μg/mL), P. aeruginosa 60811001 (MIC = 2 μg/mL), P. aeruginosa 60909001 (MIC = 2 μg/mL), P. aeruginosa 61118003 (MIC = 2 μg/mL), P. aeruginosa 70108001 (MIC = 4 μg/mL), E. coli 70329001 (MIC = 1 μg/mL), E. coli 70430001 (MIC = 1 μg/mL), E. coli 70419002 (MIC = 8 μg/mL), E. coli 70502001 (MIC = 1 μg/mL), E. coli Neumann (MIC = 2 μg/mL), E. coli 205024 (MIC = 4 μg/mL), E. coli 455/7 (MIC = 0.5 μg/mL), E. coli 8474 (MIC = 0.5 μg/mL), K. pneumoniae DSM681 (MIC = 0.25 μg/mL), K. pneumoniae 57 USA (MIC = 0.25 μg/mL), K. pneumoniae 63 (MIC = 8 μg/mL), K. pneumoniae 8085 (MIC = 0.5 μg/mL), K. pneumoniae ATCC12657 (0.5 μg/mL), K. pneumoniae ATCC27799 (MIC = 1 μg/mL), A. baumannii ICB14067 (MIC = 1 μg/mL), A. baumannii ICB14072 (MIC = 1 μg/mL), A. baumannii ICB14073 (MIC = 2 μg/mL), A. baumannii ICB9250 (MIC = 0.5 μg/mL), A. baumannii M17 (MIC = 1 μg/mL), E. cloacae 34654 (MIC = 0.25 μg/mL), E. cloacae Ha088 (MIC = 0.125 μg/mL), E. cloacae Ha089 (0.125 μg/mL), E. cloacae Ha185 (MIC = 0.5 μg/mL), E. cloacae Ha89 (MIC = 0.25 μg/mL), P. vulgaris ICB9081 (MIC = 4 μg/mL), P. vulgaris ICB9095 (MIC = 4 μg/mL), Stenotrophomonas ICB308054 (MIC = 32 μg/mL), Stenotrophomonas ICB7569 (MIC = 8 μg/mL), Stenotrophomonas ICB7789 (MIC = 32 μg/mL)

• • Hemolytic Activity

  • • [Ref.20565063]None of the tested peptides showed any hemolytic activity against human erythrocytes in the studied peptide concentration range up to 600 μg/mL.

• • Cytotoxicity

  • • [Ref.20565063] Oncocin showed no toxic effects on HeLa- or neuronal-cell-like differentiated SH-SY5Y cells at a peptide concentration of 600 μg/mL, which was about 100-fold above their MIC-values against E.coli BL21 AI.

• • Binding Target

  • Bacterial ribosome

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP29043.

DRAMP29043

• • Comment

  • Oncocin inhibits bacterial protein translation by targeting the ribosome.

• ·Literature 1

• • Title

  • Oncocin (VDKPPYLPRPRPPRRIYNR-NH2): A Novel Antibacterial Peptide Optimized against Gram-Negative Human Pathogens

• • Pubmed ID

  • 20565063

• • Reference

  • J Med Chem. 2010 Jul 22;53(14):5240-7. doi: 10.1021/jm100378b.

• • Author

  • Daniel Knappe, Stefania Piantavigna, Anne Hansen, Adam Mechler, Annegret Binas, Oliver Nolte, Lisandra L Martin, Ralf Hoffmann