General Information
-
DRAMP ID
- DRAMP29044
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Peptide Name
- Cbf-K-16 (variant of BF-30)
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Source
- Synthetic construct (Cbf-K16 is a variant of BF-30, which was found in the venom of the snake bungarus fasciatus)
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Family
- Not found
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Gene
- N/A
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Sequence
- KFFRKLKKSVKKRAKKFFKKPRVIGVSIPF
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Sequence Length
- 30
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Target Organism
-
- [Ref.31704464] Gram-negative drug-resistant clinical isolates: H. pylori SS1 (MIC = 16 μg/mL, MBC = 32 μg/mL); Gram -negative bacteria: H. pylori 26695 (MIC = 32 μg/mL, MBC = 64 μg/mL), H. pylori 11637 (MIC = 16 μg/mL, MBC = 64 μg/mL)
- [Ref.27287456] Gram-positive drug-resistant clinical isolates: MRSA strain no.1 (MIC = 32 μg/mL), MRSA strain no.2 (MIC = 16 μg/mL), S.aureus strain no.1 (MIC = 16 μg/mL), S.aureus strain no.2 (MIC = 64 μg/mL), S.aureus strain no.3 (MIC = 8 μg/mL), S.aureus strain no.4 (MIC = 16 μg/mL), S.aureus strain no.5 (MIC = 4 μg/mL), S.aureus strain no.6 (MIC = 8 μg/mL), S.aureus strain no.7 (MIC = 32 μg/mL), S.epidermidis strain no.1 (MIC = 16 μg/mL), S.epidermidis strain no.2 (MIC = 32 μg/mL);
- Gram-positive bacteria: S.aureus ATCC 25923 (MIC = 8 μg/mL);
- Gram-negative drug-resistant clinical isolates: E.coli strain no.1 (MIC = 4 μg/mL), E.coli strain no.2 (MIC = 8 μg/mL), E.coli strain no.3 (MIC = 8 μg/mL);
- Gram-negative bacteria: E.coli ATCC 25922 (MIC = 4 μg/mL)
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Hemolytic Activity
-
- [Ref.27287456]Cbf-K-16 has low hemolytic activity
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Cytotoxicity
-
- [Ref.27287456] Cbf-K-16 has low cytotoxicity.
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Binding Target
- Genomic DNA
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
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C-terminal Modification
- Free
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Nonterminal Modifications and Unusual Amino Acids
- None
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Stereochemistry
- L
-
Structure
- Not found
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP29044.
Physicochemical Information
-
Formula
- C177H295N49O33
Absent Amino Acids
- CDEHMNQTWY
Common Amino Acids
- K
Mass
- 3637.6
PI
- 12.33
Basic Residues
- 13
Acidic Residues
- 0
Hydrophobic Residues
- 12
Net Charge
- +13
-
Boman Index
- -6253
Hydrophobicity
- -0.55
Aliphatic Index
- 71.33
Half Life
-
- Mammalian:1.3 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 0
Absorbance 280nm
- 0
Polar Residues
- 3
DRAMP29044
Comments Information
Comment
- Cbf-K16 possessed effective antimicrobial and anti-inflammatory activities and downregulated the expression of adhesion- and cytotoxin-associated genes of drug-resistant H. pylori SS1, making it a potential candidate for anti-infective therapy.
Literature Information
- ·Literature 1
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Title
- Antimicrobial activities of peptide Cbf-K 16 against drug-resistant Helicobacter pylori infection in vitro and in vivo.
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Pubmed ID
- 31704464
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Reference
- Microb Pathog. 2020 Jan;138:103847. doi: 10.1016/j.micpath.2019.103847. Epub 2019 Nov 5.
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Author
- Jiang M, Ma L, Huang Y, Wu H, Dou J, Zhou C
- ·Literature 2
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Title
- The antimicrobial activity of Cbf-K16 against MRSA was enhanced by β-lactamantibiotics through cell wall non-integrity.
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Pubmed ID
- 27287456
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Reference
- Arch Pharm Res. 2016 Jul;39(7):978-88. doi: 10.1007/s12272-016-0769-x. Epub 2016 Jun 10.
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Author
- Bo Li, Wei Kang, Hanhan Liu, Yanrong Wang, Changzhong Yu, Xinyi Zhu, Jie Dou, Haibo Cai, Changlin Zhou