General Information

    • DRAMP ID

    • DRAMP29045

    • Peptide Name

    • PA2-GNU7

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • SQRKLAAKLTSKGGGRLLRPLLQLLKQKLR

    • Sequence Length

    • 30

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.31704464] Gram-positive bacteria: S. aureus (MIC = 2 μM);
      • Gram-negative bacteria: P. aeruginosa (MIC = 2 μM), E. coli (MIC = 1 μM), S. typhimurium (MIC = 2 μM);
      • Fungi: C.albicans (MIC = 8 μM)

    • Hemolytic Activity

      • [Ref.31678742]PA2-GNU7 was not toxic to human RBCs at the tested concentrations (0 - 65 μM).The haemolysis was less than 2.5 % up to the concentration of 65 μM.

    • Cytotoxicity

      • Not found

    • Binding Target

    • Outer membrane proteins (OMPs) like OprF, the major outer membrane porin of P.aeruginosa

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Helix-loop-helix structure

    • Structure Description

    • ①Molecular modelling showed that the hybrid peptide was predicted to form a helix-loop-helix structure. ②The hybrid peptide PA2-GNU7 was constructed by joining the PA2 peptide (SQRKLAAKLTSK) to GNU7 (RLLRPLLQLLKQKLR) with a short linker (Gly × 3).

    • Helical Wheel Diagram

    • DRAMP29045 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29045.

Physicochemical Information

    • Formula

    • C150H276N50O37

    • Absent Amino Acids

    • CDEFHIMNVWY

    • Common Amino Acids

    • L

    • Mass

    • 3372.15

    • PI

    • 12.49

    • Basic Residues

    • 9

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +9

    • Boman Index

    • -6270

    • Hydrophobicity

    • -0.51

    • Aliphatic Index

    • 123.67

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 6

DRAMP29045

DRAMP29045 chydropathy plot

Comments Information

    • Comment

    • The specific binding activity of PA2-GNU7 against P. aeruginosa was derived from the binding activity of PA2 peptide.

Literature Information

  • ·Literature 1

    • Title

    • Development of a novel hybrid antimicrobial peptide for targeted killing of Pseudomonas aeruginosa

    • Reference

    • Eur J Med Chem. 2020 Jan 1;185:111814. doi: 10.1016/j.ejmech.2019.111814. Epub 2019 Oct 25.

    • Author

    • Kim H, Jang JH, Kim SC, Cho JH.