General Information
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
-
Target Organism
-
- [Ref.32439180] Gram-negative bacteria: E. coli (MIC = 4 μM), P. aeruginosa (MIC = 16 μM), S. typhimurium (MIC = 4 μM)
- Gram-positive bacteria:B. subtilis (MIC = 8 μM), S. epidermidis (MIC = 8 μM), S. aureus(MIC = 16 μM)
-
Hemolytic Activity
-
- [Ref.32439180]HC10 > 256 μM against sheep RBCs.Note: HC10 is the minimum peptide concentration that caused >10% hemolysis of sheep red blood cells.
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Cytotoxicity
-
- Not found
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Binding Target
- Bacterial DNA
Structure Information
-
Linear/Cyclic
- Linear
-
N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
-
Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- α-helical (most likely)
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Structure Description
- Not found
-
Helical Wheel Diagram
-
PDB ID
- None
-
Predicted Structure
- There is no predicted structure for DRAMP29046.
Physicochemical Information
-
Formula
- C77H118N24O15
Absent Amino Acids
- CDEGHIMPQTVY
Common Amino Acids
- K
Mass
- 1619.94
PI
- 12.02
Basic Residues
- 5
Acidic Residues
- 0
Hydrophobic Residues
- 5
Net Charge
- +5
-
Boman Index
- -4216
Hydrophobicity
- -1.533
Aliphatic Index
- 40.83
Half Life
-
- Mammalian:1.1 hour
- Yeast:3 min
- E.coli:2 min
Extinction Coefficient Cystines
- 11000
Absorbance 280nm
- 1000
Polar Residues
- 2
DRAMP29046
Comments Information
Function
- Kuwasako et al. identified the C-terminal 12-residues of PAMP [PAMP(9–20)] in porcine adrenal medulla as a major endogenous active peptide of the AM precursor and demonstrated that its hypotensive activity is comparable to that of PAMP. PAMP(9-20) can acts as an potent antimicrobial peptide which is more selective than melittin, and antimicrobial action of PAMP(9-20) seems to exert via an intracellular target.
Literature Information
- ·Literature 1
-
Title
- Proadrenomedullin N-terminal 20 peptide (PAMP) and its C-terminal 12-residue peptide, PAMP(9-20): Cell selectivity and antimicrobial mechanism
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Pubmed ID
- 32439180
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Reference
- Biochem Biophys Res Commun. 2020 Jun 30;527(3):744-750. doi: 10.1016/j.bbrc.2020.04.063. Epub 2020 May 18.
-
Author
- Ajish C, Yang S, Kumar SD, Shin SY.