• • DRAMP ID

  • DRAMP29052

• • Peptide Name

  • Brevinin-1GHd

• • Source

  • Sylvirana guentheri (Gunther's frog) (Rana guentheri)

• • Family

  • Belongs to the frog skin active peptide (FSAP) family. Brevinin subfamily

• • Gene

  • N/A

• • Sequence

  • FLGALFKVASKLVPAAICSISKKC

• • Sequence Length

  • 24

• • UniProt Entry

  • A0A6M4CA40

• • Protein Existence

  • Transcript level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

• • Target Organism

  • • [Ref.32347293] Gram-positive bacteria: S. aureus (MIC = 2 μM/MBC= 4 µM), MRSA(MIC = 4 μM/MBC = 4µM);
    • Gram-negative: E. coli(MIC = 8 μM/MBC = 8 µM), P. aeruginosa(MIC = 32 μM/MBC = 32µM);
    • Fungi: C. albicans(MIC = 4 μM/MBC = 8µM）

• • Hemolytic Activity

  • • [Ref.32347293] Brevinin-1GHd displayed 13% hemolysis on horse red blood cells at its highest MIC/MBC value of the tested bacteria P. aeruginosa (32 µM). However, when the concentration increased above 64 μM, the hemolytic activity of Brevinin-1GHd also gradually increased. At the concentration of 64, 128, 256 and 512 μM, it displayed about 36%, 64%, 83% and 108% hemolysis.

• • Cytotoxicity

  • • [Ref.32347293]Brevinin-1GHd showed significant cytotoxicity toward human HMEC-1 and HaCaT cells at concentrations of 0.00001 and 0.0001 M, respectively. Brevinin-1GHd induced one cell viability of 56% on HMEC-1 and the other cell viability of 75% on HaCat.

• • Binding Target

  • Cell membrane

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • Free

• • Stereochemistry

  • L

• • Structure

  • α-helical

• • Structure Description

  • The results of CD spectroscopy revealed that Brevinin-1GHd adopt a random coil structure in an aqueous environment, while forming an alpha-helix structure in a membrane-mimetic environment.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP29052.

DRAMP29052

• • Comment

  • Brevinin-1GHd with its excellent antimicrobial and anticancer activities is a promising candidate for a novel antibiotic agent, and study of its structure activity relationships also provided a rational template for further research and peptide analog design. In the reference, the example of Brevinin-1GHd is used to challenge the importance of "Rana-Box", which appears in many AMPs originating from frogs and contains an intermolecular disulfide bridge at the C-terminus of the peptide and seven to nine amino acid residues.

• ·Literature 1

• • Title

  • Brevinin-1GHd: a novel Hylarana guentheri skin secretion-derived Brevinin-1 type peptide with antimicrobial and anticancer therapeutic potential

• • Pubmed ID

  • 32347293

• • Reference

  • Biosci Rep. 2020 May 29;40(5):BSR20200019. doi: 10.1042/BSR20200019.

• • Author

  • Jiang Y, Wu Y, Wang T, Chen X, Zhou M, Ma C, Xi X, Zhang Y, Chen T, Shaw C, Wang L.