General Information

    • DRAMP ID

    • DRAMP29055

    • Peptide Name

    • temporin G

    • Source

    • Rana temporaria (European common frog)

    • Family

    • Belongs to the frog skin active peptide (FSAP) family. Temporin subfamily.

    • Gene

    • N/A

    • Sequence

    • FFPVIGRILNGIL

    • Sequence Length

    • 13

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal, Antibiofilm

    • Target Organism

      • [Ref.33321906] S. aureus ATCC 25923 biofilm: TG was capable of killing at least 39%, 78% and 87% at 3.12, 6.25, 12.5 and 25 µM.
      • [Ref.22439858] Summary: TG had specific bactericidal activities against E. coli ATCC2592, M. luteus or S. cerevisiae.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • [Ref.33321906]TG was shown to be harmless against human keratinocytes at all concentrations tested with a slight cytotoxicity (~20% reduction of metabolically-active cells) at the highest active concentration

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Amphipathic alpha-helical (Uniprot:P79875)

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29055 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29055.

Physicochemical Information

    • Formula

    • C72H115N17O15

    • Absent Amino Acids

    • ACDEHKMQSTWY

    • Common Amino Acids

    • I

    • Mass

    • 1458.81

    • PI

    • 9.75

    • Basic Residues

    • 1

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +1

    • Boman Index

    • 1492

    • Hydrophobicity

    • 1.577

    • Aliphatic Index

    • 172.31

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 3

DRAMP29055

Comments Information

    • Function

    • Amphipathic alpha-helical antimicrobial peptide with activity against Gram-positive bacteria (M.luteus), Gram-negative bacteria (E.coli), and fungi (S.cerevisiae) (PubMed

Literature Information

  • ·Literature 1

    • Title

    • The Antimicrobial Peptide Temporin G: Anti-Biofilm, Anti-Persister Activities, and Potentiator Effect of Tobramycin Efficacy Against Staphylococcus aureus.

    • Reference

    • Int J Mol Sci. 2020 Dec 10;21(24):9410. doi: 10.3390/ijms21249410.

    • Author

    • Casciaro B, Loffredo MR, Cappiello F, Fabiano G, Torrini L, Mangoni ML
  • ·Literature 2

    • Title

    • A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes

    • Reference

    • BMC Biotechnol. 2012 Mar 23;12:10. doi: 10.1186/1472-6750-12-10.

    • Author

    • Tao Ke, Su Liang, Jin Huang, Han Mao, Jibao Chen, Caihua Dong, Junyan Huang, Shengyi Liu, Jianxiong Kang, Dongqi Liu, Xiangdong Ma