General Information

    • DRAMP ID

    • DRAMP29058

    • Peptide Name

    • P6.2

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • KGLLRKWGKKWKEFLRRVW

    • Sequence Length

    • 19

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.31778756] Gram-negative bacteria: P. aeruginosa (MIC = 16 μg/mL, MBC = 32 μg/mL);
      • Gram-positive bacteria: S. aureus (MIC = 32 µg/mL, MBC = 128 μg/mL)

    • Hemolytic Activity

      • [Ref.31778756]P6.2 exhibited a concentration dependent hemolytic activity, reaching values of 30% at the highest concentration(1024 μg/mL), but remaining below 5% until 128 μg/ml, which is fourfold or eightfold the MIC obtained for S.aureus or P.aeruginosa, respectively.

    • Cytotoxicity

      • Not found

    • Binding Target

    • Bacterial cytoplasmic membranes

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helical

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29058 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29058.

Physicochemical Information

    • Formula

    • C122H192N36O22

    • Absent Amino Acids

    • ACDHIMNPQSTY

    • Common Amino Acids

    • K

    • Mass

    • 2515.09

    • PI

    • 11.75

    • Basic Residues

    • 8

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +7

    • Boman Index

    • -4867

    • Hydrophobicity

    • -1.137

    • Aliphatic Index

    • 76.84

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 16500

    • Absorbance 280nm

    • 916.67

    • Polar Residues

    • 2

DRAMP29058

DRAMP29058 chydropathy plot

Comments Information

    • Comment

    • This new peptide could be good candidates for future drug development for anti-biofilm and anti-infective therapy.

Literature Information

  • ·Literature 1

    • Title

    • Antibacterial, anti-biofilm and in vivo activities of the antimicrobial peptides P5 and P6.2

    • Reference

    • Microb Pathog. 2020 Feb;139:103886. doi: 10.1016/j.micpath.2019.103886. Epub 2019 Nov 25.

    • Author

    • Martínez M, Polizzotto A, Flores N, Semorile L, Maffía PC