General Information

    • DRAMP ID

    • DRAMP29061

    • Peptide Name

    • GHaR7R (variant of temporin-GHaR)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • FLQRIIRALGRLF

    • Sequence Length

    • 13

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal

    • Target Organism

      • [Ref.33291521] Gram-positive bacteria: S. aureus(MIC / MBC = 3.1μM / 3.1μM), B. subtilis(MIC / MBC > 50μM / 50μM), S. mutans(MIC / MBC = 6.2μM / 12.5μM), MRSA(MIC / MBC = 3.1μM / 6.2μM), MRSA-1(MIC /MBC = 6.2μM / 25μM), MRSA-2(MIC / MBC = 6.2μM / 50μM), MRSA-3(MIC / MBC = 6.2μM / 25μM)
      • Gram-negative bacteria: E. coli(MIC / MBC = 3.1μM / 12.5μM), D31(MIC /MBC = 12.5μM / 50μM), P. aeruginosa(MIC / MBC > 50μM / 50μM), PAO1(MIC / MBC > 50μM / 50μM)
      • Fungi: C. albicans(MIC / MBC = 25μM / 25μM)

    • Hemolytic Activity

      • [Ref.33291521]HL50 = 10μM against human red blood cells (hRBCs)

    • Cytotoxicity

      • [Ref.33291521]GHaR6R induced a cell viability of 62%, 50% and 39% to human oral epithelial cells at 3.1μM, 6.2μM and 12.5μM.

    • Binding Target

    • Cell membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helical

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29061 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29061.

Physicochemical Information

    • Formula

    • C76H127N23O15

    • Absent Amino Acids

    • CDEHKMNPSTVWY

    • Common Amino Acids

    • LR

    • Mass

    • 1602.99

    • PI

    • 12.3

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +3

    • Boman Index

    • -1699

    • Hydrophobicity

    • 0.8

    • Aliphatic Index

    • 157.69

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 1

DRAMP29061

Comments Information

    • Comment

    • GHaR6R not only exerted antimicrobial activity by destroying cell membranes of planktonic bacteria, but also exhibited anti-biofilm activity. However, the hemolysis and cytotoxicity to HOECs of GHaR7R is too strong to make it not suitable be a good antimicrobial candidate

Literature Information

  • ·Literature 1

    • Title

    • Temporin-Like Peptides Show Antimicrobial and Anti-Biofilm Activities against Streptococcus mutans with Reduced Hemolysis

    • Reference

    • Molecules. 2020 Dec 4;25(23):5724. doi: 10.3390/molecules25235724.

    • Author

    • Wei H, Xie Z, Tan X, Guo R, Song Y, Xie X, Wang R, Li L, Wang M, Zhang Y