• • DRAMP ID

  • DRAMP29065

• • Peptide Name

  • AMPR-11(derived from Romo1)

• • Source

  • Homo sapiens (Human)

• • Family

  • Belongs to the MGR2 family.

• • Gene

  • ROMO1

• • Sequence

  • KTMMQSGGTFGTFMAIGMGIR

• • Sequence Length

  • 21

• • UniProt Entry

  • P60602

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram-, Anti-Gram+

• • Target Organism

  • • [Ref.33291307] Gram-positive bacteria: S. aureus(MBC=100 μg/mL), B. subtilis(MBC=90 μg/mL), E. faecium(MBC=85 μg/mL), S. sindenensis(MBC=95 μg/mL), E. faecalis(MBC=85 μg/mL), S. pneumoniae(MBC=100 μg/mL)
    • Gram-negative bacteria: E. coli(MBC=85 μg/mL), P. aeruginosa(MBC=100 μg/mL), K. pneumoniae(MBC=100 μg/mL), A. baumannii(MBC=100 μg/mL), E. aerogenes(MBC=90 μg/mL)
    • Multi-drug resistant bacteria: Methicillin-resistant S. aureus(MBC=100 μg/mL), Carbapenem-resistant P. aeruginosa(MBC=110 μg/mL), Carbapenem-resistant A. baumannii(MBC=110 μg/mL), Carbapenem-resistant K. pneumoniae(MBC=100 μg/mL), Vancomycin-resistant S. aureus(MBC=120 μg/mL), Vancomycin-resistant E. faecium(MBC=100 μg/mL )

• • Hemolytic Activity

  • • [Ref.33291307]At the concentration of 32 μg/mL, AMPR-11 induced a hemolysis of 20% on mouse red blood cells; At the concentration of 256 μg/mL, AMPR-11 induced a hemolysis of 23%.

• • Cytotoxicity

  • • [Ref.33291307] ①The cell viability of HeLa cells was 90%, 87%, 83% and 79% at the concentration of 32, 64, 128 and 256 μg/mL. ②The cell viability of HEK293 cells was 86%, 83%, 80% and 78% at the concentration of 32, 64, 128 and 256 μg/mL. ③The cell viability of HeLa cells was 89%, 86%, 83% and 80% at the concentration of 32, 64, 128 and 256 μg/mL.

• • Binding Target

  • Bacterial membrane

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • α-helical

• • Structure Description

  • AMPR-11 formed a random coil structure in distilled water but formed an ordered conformation in 50% hexafluoro-2-propanol (HFIP), which has been used for implementing a membrane-mimic environment

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP29065.

DRAMP29065

• • Comment

  • AMP derived from Romo1 (AMPR-11) is a promising agent for treatment of sepsis caused by MDR bacteria, and AMPR-11 could be combined with other potential antibiotics for clinical treatment.

• ·Literature 1

• • Title

  • Romo1-Derived Antimicrobial Peptide Is a New Antimicrobial Agent against Multidrug-Resistant Bacteria in a Murine Model of Sepsis

• • Pubmed ID

  • 32291307

• • Reference

  • mBio. 2020 Apr 14;11(2):e03258-19. doi: 10.1128/mBio.03258-19.

• • Author

  • Lee HR, You DG, Kim HK, Sohn JW, Kim MJ, Park JK, Lee GY, Yoo YD