• • DRAMP ID

  • DRAMP29066

• • Peptide Name

  • Marine peptide-N6

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

  • N/A

• • Sequence

  • GFAWNVCVYRNGVRVCHRRAN

• • Sequence Length

  • 21

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Synthetic form

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram-, Anti-Gram+

• • Target Organism

  • • [Ref.33348729] Gram-negative bacteria: Edwardsiella tarda(MIC = 6.4 μg/mL), Aeromonas veronii(MIC = 6.4 μg/mL), Escherichia coli CVCC195(MIC = 0.5 μg/mL), E. coli CVCC1515(MIC = 1 μg/mL), E. coli CVCC25922(MIC = 0.25 μg/mL), E. coli CVCCO157(MIC = 0.5 μg/mL), Salmonella typhimurium CVCC533(MIC = 1 μg/mL), S. typhimurium ATCC14028(MIC = 2 μg/mL), S. enteritidis CVCC3377(MIC = 0.25 μg/mL), S. pullorum CVCC1802(MIC = 0.5 μg/mL), S. pullorum CVCC1789(MIC = 0.5 μg/mL), Pseudomonas aeruginosa CICC21630(MIC = 4 μg/mL).
    • Gram-positive bacteria: Staphylococcus aureus ATCC43300(MIC = 16 μg/mL), S. aureus ATCC546(MIC = 4 μg/mL), S. aureus ATCC25923(MIC = 0.25 μg/mL), S. hyicus 437-2(MIC = 64 μg/mL), S. hyicus 15095(MIC = 16 μg/mL).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • • [Ref.33348729]Had very low cytotoxicity to mouse peritoneal RAW 264.7 macrophages (cell survival rate >93.35%)

• • Binding Target

  • Lipopolysaccharide (LPS) and genomic DNA

• • Linear/Cyclic

  • Cyclic

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • There is a disulfide bond between Cys7 and Cys16

• • Stereochemistry

  • L

• • Structure

  • β-turn, coil and antiparallel sheet

• • Structure Description

  • ①The structure analysis showed that N6 have one rigid disulfide bridge Cys7-Cys16, which links the two β-strands (Cys3-Asn10 and Arg13-Cys20) and forms an anti-parallel β-sheet. ②The secondary structures of N6NH2 and N6 in ddH2O were characterized by a coil and antiparallel strand or β-turn with a negative minimum at 200 nm. N6NH2 showed a more significant increase in α-helix and antiparallel strand in SDS solution than N6. Similarly, the CD spectrum of N6 and N6NH2 in 50% TFE showed one negative dichroic band at approximately 205 nm, and the positive maximum at 180 nm (strong) and at 230 nm

• • Helical Wheel Diagram

  • 

• • PDB ID

  • 5Y0H
• 5Y0H-> 

• Predicted Structure

• There is no predicted structure for DRAMP29066.

DRAMP29066

• • Comment

  • N6NH2 and N6 are different from common antimicrobial peptides in the secondary structure. N6 has good antimicrobial activity.

• ·Literature 1

• • Title

  • Improved Stability and Activity of a Marine Peptide-N6NH2 against Edwardsiella tarda and Its Preliminary Application in Fish

• • Pubmed ID

  • 33348729

• • Reference

  • Mar Drugs. 2020 Dec 17;18(12):650. doi: 10.3390/md18120650.

• • Author

  • Han H, Li T, Wang Z, Teng D, Mao R, Hao Y, Yang N, Wang X, Wang J.