General Information

    • DRAMP ID

    • DRAMP29069

    • Peptide Name

    • CPF-2-2 (Variant of CPF-C1)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • kFwSLLkKAlrLwAkVL

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • [Ref.32590058] Gram-positive: S. aureus ATCC 25923 (MIC = 8 μg/mL, MIC = 128 μg/mL in 10% serum, MIC = 8 μg/mL in 150 mM, MBIC50 = 16 μg/mL, MBIC90 = 16 μg/mL, MBEC = 16 μg/mL), MRSA ATCC 33591 (MIC = 8 μg/mL), MRSA 054 isolated from clinics (MIC = 8 μg/mL), MRSA 023 isolated from clinics (MIC = 8 μg/mL), MRSA 113 isolated from clinics (MIC = 8 μg/mL), MRSA 048 isolated from clinics (MIC = 8 μg/mL), MRSA 002 isolated from clinics (MIC = 8 μg/mL), MRSA 074 isolated from clinics (MIC = 8 μg/mL), MRSA 051 isolated from clinics (MIC = 16 μg/mL), MRSA 052 isolated from clinics (MIC = 8 μg/mL), MRSA 075 isolated from clinics (MIC = 8 μg/mL), MRSA 071 isolated from clinics (MIC = 8 μg/mL), MRSA 936 isolated from clinics (MIC = 8 μg/mL), S. aureus 725 (MIC = 16 μg/mL), S. aureus 794 (MIC = 8 μg/mL)

    • Hemolytic Activity

      • [Ref.32590058]It showed almost no hemolytic activity, even at concentrations up to 128 μg/ml

    • Cytotoxicity

      • Not found

    • Binding Target

    • Bacterial membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • Introduction of D-amino acid(s). (Look at 'Stereochemistry')

    • Stereochemistry

    • Mixed (D-Lys1, D-Trp3, D-Lys7, D-Leu10, D-Arg11, D-Trp13 and D-Lys15)

    • Structure

    • α-helical (most likely)

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29069 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29069.

Physicochemical Information

    • Formula

    • C105H170N26O19

    • Absent Amino Acids

    • CDEGHIMNPQTY

    • Common Amino Acids

    • L

    • Mass

    • 2100.67

    • PI

    • 11.33

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Net Charge

    • +5

    • Boman Index

    • -62

    • Hydrophobicity

    • 0.406

    • Aliphatic Index

    • 143.53

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 11000

    • Absorbance 280nm

    • 687.5

    • Polar Residues

    • 1

DRAMP29069

Comments Information

    • Comment

    • CPF-2 has potential as a lead compound to treat infections caused by S. aureus and MRSA, including the associated biofilms.

Literature Information

  • ·Literature 1

    • Title

    • CPF-C1 analog with effective antimicrobial and antibiofilm activities against Staphylococcus aureus including MRSA

    • Reference

    • Biochimie. 2020 Sep;176:1-11. doi: 10.1016/j.biochi.2020.06.003. Epub 2020 Jun 23.

    • Author

    • Xie J, Li Y, Guo X, Rao J, Yan T, Mou L, Wu X, Xie X, Yang W, Zhang B.