General Information

    • DRAMP ID

    • DRAMP29070

    • Peptide Name

    • CPF-13 (Variant of CPF-C1)

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • GFGSLLGKALRLWKKVL

    • Sequence Length

    • 17

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Synthetic form

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • [Ref.32590058] Gram-positive: S. aureus ATCC 25923 (MIC = 4 μg/mL, MIC = 8 μg/mL in 10% serum, MIC = 4 μg/mL in 150 mM, MBIC50 = 4 μg/mL, MBIC90 = 8 μg/mL, MBEC = 8 μg/mL), MRSA ATCC 33591 (MIC = 8 μg/mL), MRSA 054 isolated from clinics (MIC = 8 μg/mL), MRSA 023 isolated from clinics (MIC = 8 μg/mL), MRSA 113 isolated from clinics (MIC = 8 μg/mL), MRSA 048 isolated from clinics (MIC = 8 μg/mL), MRSA 002 isolated from clinics (MIC = 8 μg/mL), MRSA 074 isolated from clinics (MIC = 8 μg/mL), MRSA 051 isolated from clinics (MIC = 8 μg/mL), MRSA 052 isolated from clinics (MIC = 8 μg/mL), MRSA 075 isolated from clinics (MIC = 8 μg/mL), MRSA 071 isolated from clinics (MIC = 8 μg/mL), MRSA 936 isolated from clinics (MIC = 8 μg/mL), S. aureus 725 (MIC = 8 μg/mL), S. aureus 794 (MIC = 8 μg/mL)

    • Hemolytic Activity

      • [Ref.32590058]It showed significant toxicity at high concentrations. At the concentration of 16, 32, 64 and 128 μg/mL, CPF-13 displayed 2%, 10%, 72% and 72% hemolysis, respectively.

    • Cytotoxicity

      • Not found

    • Binding Target

    • Bacterial membrane

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • α-helical

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29070 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29070.

Physicochemical Information

    • Formula

    • C91H152N24O19

    • Absent Amino Acids

    • CDEHIMNPQTY

    • Common Amino Acids

    • L

    • Mass

    • 1886.36

    • PI

    • 11.26

    • Basic Residues

    • 4

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +4

    • Boman Index

    • 361

    • Hydrophobicity

    • 0.512

    • Aliphatic Index

    • 137.65

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 5500

    • Absorbance 280nm

    • 343.75

    • Polar Residues

    • 4

DRAMP29070

Comments Information

    • Comment

    • CPF-13 showed improved antimicrobial activities against S. aureus and stabilities in serum and salt solution.

Literature Information

  • ·Literature 1

    • Title

    • CPF-C1 analog with effective antimicrobial and antibiofilm activities against Staphylococcus aureus including MRSA

    • Reference

    • Biochimie. 2020 Sep;176:1-11. doi: 10.1016/j.biochi.2020.06.003. Epub 2020 Jun 23.

    • Author

    • Xie J, Li Y, Guo X, Rao J, Yan T, Mou L, Wu X, Xie X, Yang W, Zhang B.