General Information
-
DRAMP ID
- DRAMP29071
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Peptide Name
- CPF-14 (Variant of CPF-C1)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- N/A
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Sequence
- GFGSLLGKALRLwKkVL
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Sequence Length
- 17
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+
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Target Organism
-
- [Ref.32590058] Gram-positive: S. aureus ATCC 25923 (MIC = 8 μg/mL, MIC = 4 μg/mL in 10% serum, MIC = 8 μg/mL in 150 mM, MBIC50 = 16 μg/mL, MBIC90 = 16 μg/mL, MBEC = 32 μg/mL), MRSA ATCC 33591 (MIC = 8 μg/mL), MRSA 054 isolated from clinics (MIC = 8 μg/mL), MRSA 023 isolated from clinics (MIC = 8 μg/mL), MRSA 113 isolated from clinics (MIC = 8 μg/mL), MRSA 048 isolated from clinics (MIC = 8 μg/mL), MRSA 002 isolated from clinics (MIC = 8 μg/mL), MRSA 074 isolated from clinics (MIC = 8 μg/mL), MRSA 051 isolated from clinics (MIC = 8 μg/mL), MRSA 052 isolated from clinics (MIC = 8 μg/mL), MRSA 075 isolated from clinics (MIC = 8 μg/mL), MRSA 071 isolated from clinics (MIC = 8 μg/mL), MRSA 936 isolated from clinics (MIC = 8 μg/mL), S. aureus 725 (MIC = 8 μg/mL), S. aureus 794 (MIC = 8 μg/mL)
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Hemolytic Activity
-
- [Ref.32590058]It exhibited low hemolytic activity against mouse RBCs. CPF-14 displayed 4% and 13% hemolysis at the concentration of 64 and 128 μg/mL
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Cytotoxicity
-
- Not found
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Binding Target
- Bacterial membrane
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- Introduction of D-amino acid(s). (Look at 'Stereochemistry')
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Stereochemistry
- Mixed (D-Trp13 and D-Lys15)
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Structure
- α-helical
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Structure Description
- Not found
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Helical Wheel Diagram
-
PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP29071.
Physicochemical Information
-
Formula
- C91H152N24O19
Absent Amino Acids
- CDEHIMNPQTY
Common Amino Acids
- L
Mass
- 1886.36
PI
- 11.26
Basic Residues
- 4
Acidic Residues
- 0
Hydrophobic Residues
- 9
Net Charge
- +4
-
Boman Index
- 361
Hydrophobicity
- 0.512
Aliphatic Index
- 137.65
Half Life
-
- Mammalian:30 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 5500
Absorbance 280nm
- 343.75
Polar Residues
- 4
DRAMP29071
Comments Information
Comment
- CPF-14 showed improved antimicrobial activities against S. aureus and stabilities in serum and salt solution.
Literature Information
- ·Literature 1
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Title
- CPF-C1 analog with effective antimicrobial and antibiofilm activities against Staphylococcus aureus including MRSA
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Pubmed ID
- 32590058
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Reference
- Biochimie. 2020 Sep;176:1-11. doi: 10.1016/j.biochi.2020.06.003. Epub 2020 Jun 23.
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Author
- Xie J, Li Y, Guo X, Rao J, Yan T, Mou L, Wu X, Xie X, Yang W, Zhang B.