General Information

    • DRAMP ID

    • DRAMP29073

    • Peptide Name

    • CF-14

    • Source

    • Catfish skin mucus

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • RIVELTLPRVSVRL

    • Sequence Length

    • 14

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • [Ref.32205189] Gram-negative bacteria: E.coli (MIC = 31.3 μg/mL);
      • [Ref.31291603]Gram-positive bacteria: S. putrefaciens (MIC = 62.5 μg/mL)

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • Not found

    • Binding Target

    • Bacterial DNA

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • It formed α-helix in PBS with 2.5% SDS but exhibited low α-content in PBS only.

    • Structure Description

    • The CD spectra exhibited low α-helical content in PBS only. However, in PBS with 2.5% SDS, the CD spectra for CF-14 showed double-negative bands at 208 nm and 222 nm, indicating that CF-14 formed an α-helical structure under membrane-mimetic conditions.

    • Helical Wheel Diagram

    • DRAMP29073 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29073.

Physicochemical Information

    • Formula

    • C74H135N23O19

    • Absent Amino Acids

    • ACDFGHKMNQWY

    • Common Amino Acids

    • LRV

    • Mass

    • 1651.03

    • PI

    • 11.7

    • Basic Residues

    • 3

    • Acidic Residues

    • 1

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +2

    • Boman Index

    • -2574

    • Hydrophobicity

    • 0.6

    • Aliphatic Index

    • 173.57

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 2

DRAMP29073

Comments Information

    • Comment

    • These results strongly indicated that CF-14 could be a potential novel antibiotic agent against E.coli. Hence, understanding the antibacterial mechanism and analyzing the amino acid sequence of CF-14 can aid in designing effective antimicrobial agents.

Literature Information

  • ·Literature 1

    • Title

    • Antibacterial activity and mechanism of the cell-penetrating peptide CF-14 on the gram-negative bacteria, Escherichia coli.

    • Reference

    • Fish Shellfish Immunol. 2020 May;100:489-495. doi: 10.1016/j.fsi.2020.03.038. Epub 2020 Mar 20.

    • Author

    • Li T, Liu Q, Chen H, Li J
  • ·Literature 2

    • Title

    • Characterization and antimicrobial mechanism of CF-14, a new antimicrobial peptide from the epidermal mucus of catfish

    • Reference

    • Fish Shellfish Immunol. 2019 Sep;92:881-888. doi: 10.1016/j.fsi.2019.07.015. Epub 2019 Jul 7.

    • Author

    • Tingting Li, Quanwei Liu, Dangfeng Wang, Jianrong Li