General Information
-
DRAMP ID
- DRAMP29077
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Peptide Name
- dCATH 14 (modified from dCATH)
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Source
- Synthetic construct
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Family
- Not found
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Gene
- N/A
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Sequence
- VPLAIKIYRAWKRR
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Sequence Length
- 14
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UniProt Entry
- No entry found
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Protein Existence
- Synthetic form
Activity Information
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-, Anti-Gram+
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Target Organism
-
- [Ref.32698035] Gram-positive bacteria: S. aureus KCTC 1621 (MIC = 8 μM), S. epidermidis KCTC 1917 (MIC = 16 μM), B. subtilis KCTC 3068 (MIC = 4 μM);
- Resistant Gram-positive bacteria: MRSA CCARM 3089 (MIC = 32 μM), MRSA CCARM 3090 (MIC = 32 μM), MRSA CCARM 3095 (MIC = 32 μM), VREF ATCC 51559(MIC = 16 μM);
- Gram-negative bacteria: E. coli KCTC 1682 (MIC = 16 μM), P. aeruginosa KCTC 1637(MIC = 8 μM), S. typhimurium KCTC 1926 (MIC = 8 μM);
- Resistant Gram-negative bacteria: MDRPAd CCARM 2095 (MIC = 32 μM), MDRPA CCARM 2109 (MIC = 32 μM);
- Yeast: C. albicans KCTC 7965 (MIC = 16 μM), C. albicans KCTC 7121 (MIC = 16 μM).
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Hemolytic Activity
-
- [Ref.32698035] HC10 > 256 μM. Note: HC10 is the peptide concentration that caused 10% hemolysis of sheep red blood cells (sRBCs).
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Cytotoxicity
-
- [Ref.32698035] At the concentration of 1.25, 2.5, 5, 10, 20, 40, 80 μM, the cell survival of dCATH 14 against human bone marrow SH-SY5Y cells is 103%, 101%, 100%, 100%, 91%, 74%, 70%
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Binding Target
- Not found
Structure Information
-
Linear/Cyclic
- Linear
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N-terminal Modification
- Free
-
C-terminal Modification
- Amidation
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Nonterminal Modifications and Unusual Amino Acids
- None
-
Stereochemistry
- L
-
Structure
- Unknown
-
Structure Description
- Initially, four analog peptides were designed by truncating two, four, six, and eight amino acids at the N-terminal ends of dCATH peptide. dCATH and its truncated analogs could not adopt the amphipathic α-helical structure since the hydrophobic and charged residues were dispersed all around the molecule.
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Helical Wheel Diagram
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PDB ID
- None
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Predicted Structure
- There is no predicted structure for DRAMP29077.
Physicochemical Information
-
Formula
- C84H140N26O16
Absent Amino Acids
- CDEFGHMNQST
Common Amino Acids
- R
Mass
- 1770.2
PI
- 11.73
Basic Residues
- 5
Acidic Residues
- 0
Hydrophobic Residues
- 7
Net Charge
- +5
-
Boman Index
- -3125
Hydrophobicity
- -0.321
Aliphatic Index
- 118.57
Half Life
-
- Mammalian:100 hour
- Yeast:>20 hour
- E.coli:>10 hour
Extinction Coefficient Cystines
- 6990
Absorbance 280nm
- 537.69
Polar Residues
- 1
DRAMP29077
Comments Information
Comment
- The peptide induces potent antimicrobial activity.
Literature Information
- ·Literature 1
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Title
- Antimicrobial and anti-inflammatory activities of short dodecapeptides derived from duck cathelicidin: Plausible mechanism of bactericidal action and endotoxin neutralization
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Pubmed ID
- 32698035
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Reference
- Eur J Med Chem. 2020 Oct 15;204:112580. doi: 10.1016/j.ejmech.2020.112580. Epub 2020 Jul 16.
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Author
- Kumar SD, Shin SY