• • DRAMP ID

  • DRAMP29102

• • Peptide Name

  • Hc-CATH

• • Source

  • Hydrophis cyanocinctus

• • Family

  • Not found

• • Gene

  • Not found

• • Sequence

  • KFFKRLLKSVRRAVKKFRKKPRLIGLSTLL

• • Sequence Length

  • 30

• • UniProt Entry

  • No entry found

• • Protein Existence

  • Not found

• • Biological Activity

  • Antimicrobial, Antibacterial,Anti-Gram+, Anti-Gram-, Anti-cancer, Antifungal

• • Target Organism

  • • [Ref.26013823]Gram-negative bacteria:Escherichia coli ATCC 25922(MIC=2.34 µg/ml); Escherichia coli ATCC 25922(400 Mm NaCl,MIC=4.69 µg/ml); Escherichia coli 1(MIC=2.34 µg/ml);Escherichia coli 2(MIC=2.34 µg/ml); Escherichia coli 3(MIC=2.34 µg/ml); Escherichia coli 4(MIC=9.38 µg/ml); Shigella dysenteriae(MIC=0.59 µg/ml); Klebsiella pneumoniae 1(MIC=37.50 µg/ml); Klebsiella pneumoniae 2(MIC=4.69 µg/ml); Klebsiella pneumoniae 3(MIC=9.38 µg/ml); Klebsiella pneumoniae 4(MIC=9.38 µg/ml); Klebsiella pneumoniae 5(MIC=18.75 µg/ml); Klebsiella pneumoniae 6(MIC=37.50 µg/ml); Klebsiella pneumoniae 7(MIC=37.50 µg/ml); Klebsiella pneumoniae 8(MIC=75.00 µg/ml); Serratia marcescens(MIC>200 µg/ml); Klebsiella oxytoca(MIC=4.69 µg/ml); Proteus vulgaris(MIC>200 µg/ml); Proteus mirabilis(MIC=4.69 µg/ml); Acinetobacter baumannii 1(MIC>200 µg/ml); Acinetobacter baumannii 2(MIC>200 µg/ml); Stenotrophomonas maltophilia(MIC>200 µg/ml); Stenotrophomonas maltophilia 2(MIC=9.38 µg/ml); Pseudomonas aeruginosa ATCC 27853(MIC=18.75 µg/ml); Pseudomonas aeruginosa 1 (MIC=37.50 µg/ml ); Pseudomonas aeruginosa(MIC>200 µg/ml); Salmonella paratyphi A(MIC=4.69 µg/ml);
    • Gram-positive bacteria:Staphylococcus aureus ATCC 25923(MIC=4.69 µg/ml); Staphylococcus aureus 1(MIC>200 µg/ml ); Staphylococcus aureus 2(MIC>200 µg/ml ); Staphylococcus aureus 3(MIC>200 µg/ml ); Staphylococcus aureus 4(MIC=4.69 µg/ml); Staphylococcus aureus 5(MIC=4.69 µg/ml); Bacillus cereus(MIC=9.38 µg/ml); Bacillus subtilis(MIC=75.00 µg/ml); Enterococcus faecium(MIC=37.50 µg/ml); Nocardia asteroides(MIC=9.38 µg/ml); Enterococcus faecalis(MIC>200 µg/ml); Staphylococcus epidermidis(MIC>200 µg/ml);
    • Fungi:Candida albicans 1(MIC=4.69 µg/ml); Candida albicans 2(MIC=4.69 µg/ml); Candida albicans 3(MIC=4.69 µg/ml); Candida albicans 4(MIC=4.69 µg/ml); Candida albicans 5(MIC=2.34 µg/ml); Candida albicans 6(MIC=2.34 µg/ml); Candida glabrata 1(MIC=2.34 µg/ml); Candida glabrata(MIC>200 µg/ml); Cryptococcus neoformans(MIC>200 µg/ml); Arcyria cinerea(MIC=9.38 µg/ml);
    • Pathogenic bacteria:Aeromonas sobria(MIC=2.34 µg/ml); Aeromonas hydrophila(MIC=2.34 µg/ml); Aeromonas veronii(MIC=2.34 µg/ml); Vibrio vulnificus(MIC=4.69 µg/ml ); Vibrio harveyi(MIC=9.38 µg/ml); Vibrio fluvialis(MIC=4.69 µg/ml); Vibrio alginolyticus(MIC=4.69 µg/ml); Vibrio parahaemolyticus(MIC=9.38 µg/ml); Vibrio splendidus(MIC=2.34 µg/ml); Vibrio anguillarum(MIC=18.75 µg/ml); Edwardsiella tarda(MIC=2.34 µg/ml).

• • Hemolytic Activity

  • • [Ref.26013823]5.25% Hemolysis against Human erythrocytes at 200 µg/ml(55.12 μM).

• • Cytotoxicity

  • • [Ref.26013823]4.70% cell death at 200 µg/ml against Human hepatocellular carcinoma HepG2; 3.63% cell death at 200 µg/ml against Human prostate adenocarcinoma PC-3; 1.30% cell death at 200 µg/ml against Mouse fibroblasts L929.

• • Binding Target

  • liposomes

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Free

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Alpha helix,β-sheet,random coil

• • Structure Description

  • The CD spectra in H2O adopts a random-coil conformation. In the membrane-mimetic environments of SDS/H2O solutions (30–120 mm), the secondary structure components of Hc-CATH dissolved in 60 mm SDS/H2O were calculated as 59.9% α-helix, 16% β-sheet, 0% β-turn, and 24% random coil.When dissolved in SDS/H2O solutions with serial concentrations of NaCl (at 0, 100, 200, and 400 mm), the α-helix contents decreased slightly from 59.9 to 33.8% as the NaCl concentration increased. Simultaneously, the contents of the β-turn (0 to 14.9%) and random coil (24 to 30.5%) increased slightly.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP29102.

DRAMP29102

• • Function

  • The microbial killing activity of Hc-CATH is executed through the disruption of cell membrane and lysis of bacterial cells. Antimicrobial activity of Hc-CATH in the presence of a high concentration of sodium chlorideIn addition is strong. Hc-CATH exhibited potent anti-inflammatory activity by inhibiting the LPS-induced production of nitric oxide (NO) and pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6.

• ·Literature 1

• • Title

  • Identification and Characterization of the First Cathelicidin from Sea Snakes with Potent Antimicrobial and Anti-inflammatory Activity and Special Mechanism.

• • Pubmed ID

  • 26013823

• • Reference

  • J Biol Chem. 2015 Jul 3;290(27):16633-52.

• • Author

  • Wei L, Gao J, Zhang S, Wu S, Xie Z, Ling G, Kuang YQ, Yang Y, Yu H, Wang Y.