• • DRAMP ID

  • DRAMP29104

• • Peptide Name

  • Mastoparan-C(MP-C)

• • Source

  • Vespa crabro

• • Family

  • Not found

• • Gene

  • N/A

• • Sequence

  • LNLKALLAVAKKIL

• • Sequence Length

  • 14

• • UniProt Entry

  • P01516

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial,Anti-Gram+, Anti-Gram-, Anti-cancer,Antifungal

• • Target Organism

  • • [Ref.29904274]Gram-positive bacteria:Staphylococcus aureus NCTC 10788(MIC=2 µM); Staphylococcus aureus NCTC 10788(MBC=2 µM); Staphylococcus aureus ATCC 12493(MRSA,MIC=4 µM); Staphylococcus aureus ATCC 12493(MRSA,MBC=4 µM); Enterococcus faecalis NCTC 12697(MIC=8 µM); Enterococcus faecalis NCTC 12697(MBC=8 µM);
    • Gram-negative bacteria:Escherichia coli NCTC 10418(MIC=4 µM); Escherichia coli NCTC 10418(MBC=8 µM); Pseudomonas aeruginosa ATCC 27853(MIC=8 µM); Pseudomonas aeruginosa ATCC 27853(MBC=16 µM); Fungi:Candida albicans NCTC 1467(MIC=4 µM); Candida albicans NCTC 1467(MBC=4 µM);
    • Cancer:Human squamous lung carcinoma NCI-H157(IC50=13.57 µM);Human breast adenocarcinoma MDA-MB-435S(IC50=27.70 µM);Human prostate adenocarcinoma PC-3(IC50=6.29 µM);Human glioblastoma U251-MG(IC50=36.65 µM); Human breast adenocarcinoma MCF-7(IC50=25.27 µM).
    • [Ref.33285267]Gram-positive bacteria:Staphylococcus aureus ATCC 25923(MIC=4 µM); Bacillus subtilis ATCC 23857(MIC=4 µM);
    • Gram-negative bacterial:Escherichia coli ATCC 25922(4.5 mM KCl and 0.004 mM FeCl3,MIC=4 µM); Pseudomonas aeruginosa ATCC 9027(4.5 mM KCl,MIC=8 µM); Klebsiella pneumoniae ATCC 700603(MIC=8 µM); Escherichia coli ATCC 25922(NaCl/MgCl2=150mM/1mM,MIC=4 µM); Pseudomonas aeruginosa ATCC 9027(NaCl/MgCl2=150mM/1mM,MIC=16 µM); Pseudomonas aeruginosa ATCC 9027(0.004mM FeCl3,MIC=4 µM);Escherichia coli(Rifampin-resistant strain,MIC=4 µM).

• • Hemolytic Activity

  • • [Ref.29904274]50% hemolysis against horse erythrocytes at 40.11 µM.
    • [Ref.33285267]10% hemolysis against mouse erythrocytes at 64 µM; 60% hemolysis against mouse erythrocytes at 256 µM.

• • Cytotoxicity

  • • [Ref.29904274]Cytotoxicity:Human microvascular endothelial cells HMEC-1(IC50=57.15 µM).
    • [Ref.33285267]90% Killing against Human embryonic kidney HEK293T cells at 128 µM; Human embryonic kidney HEK293T cells(IC50=16 µM).

• • Binding Target

  • liposomes

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Alpha helix,random coil

• • Structure Description

  • The CD spectra in water adopts a random-coil conformation,24.85% α-helix was determined in 50% TFE.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP29104.

DRAMP29104

• • Function

  • The peptide was tolerant in the presence of physiological salts.MP-C lost antimicrobial activities after incubation with 20 μg/mL trypsin or chymotrypsin,indicating moderate protease resistance.

• ·Literature 1

• • Title

  • Newly designed antimicrobial peptides with potent bioactivity and enhanced cell selectivity prevent and reverse rifampin resistance in Gram-negative bacteria.

• • Pubmed ID

  • 33285267

• • Reference

  • Eur J Pharm Sci. 2021 Mar 1;158:105665.

• • Author

  • Zhu N, Zhong C, Liu T, Zhu Y, Gou S, Bao H, Yao J, Ni J.

• ·Literature 2

• • Title

  • Evaluation of the bioactivity of a mastoparan peptide from wasp venom and of its analogues designed through targeted engineering.

• • Pubmed ID

  • 29904274

• • Reference

  • Int J Biol Sci. 2018 Apr 25;14(6):599-607.

• • Author

  • Chen X, Zhang L, Wu Y, Wang L, Ma C, Xi X, Bininda-Emonds ORP, Shaw C, Chen T, Zhou M.