• • DRAMP ID

  • DRAMP29106

• • Peptide Name

  • t Mastoparan-C(tMP-C, Tat (49-57)-Mastoparan-C)

• • Source

  • Synthetic construct

• • Family

  • Not found

• • Gene

  • N/A

• • Sequence

  • RKKRRQRRRLNLKALLAVAKKIL

• • Sequence Length

  • 23

• • UniProt Entry

  • P01516,Q76PP9

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial,Anti-Gram+, Anti-Gram-, Anti-cancer,Antifungal

• • Target Organism

  • • [Ref.29904274]Gram-positive bacteria:Staphylococcus aureus NCTC 10788(MIC=4 µM); Staphylococcus aureus NCTC 10788(MBC=4 µM); Staphylococcus aureus ATCC 12493(MRSA,MIC=4 µM); Staphylococcus aureus ATCC 12493(MRSA,MBC=8 µM); Enterococcus faecalis NCTC 12697(MIC=8 µM); Enterococcus faecalis NCTC 12697(MBC=16 µM);
    • Gram-negative bacteria:Escherichia coli NCTC 10418(MIC=2 µM); Escherichia coli NCTC 10418(MBC=2 µM); Pseudomonas aeruginosa ATCC 27853(MIC=4 µM); Pseudomonas aeruginosa ATCC 27853(MBC=4 µM);
    • Fungi:Candida albicans NCTC 1467(MIC=2 µM); Candida albicans NCTC 1467(MBC=2 µM);
    • Cancer:Human squamous lung carcinoma NCI-H157(IC50=2.79 µM);Human breast adenocarcinoma MDA-MB-435S(IC50=3.86 µM);Human prostate adenocarcinoma PC-3(IC50=3.86 µM);Human glioblastoma U251-MG(IC50=3.36 µM); Human breast adenocarcinoma MCF-7(IC50=3.70 µM).

• • Hemolytic Activity

  • • [Ref.29904274]50% hemolysis against horse erythrocytes at 77.94 µM.

• • Cytotoxicity

  • • [Ref.29904274]Cytotoxicity:Human microvascular endothelial cells HMEC-1(IC50=9.18 µM).

• • Binding Target

  • liposomes

• • Linear/Cyclic

  • Linear

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Amidation

• • Nonterminal Modifications and Unusual Amino Acids

  • None

• • Stereochemistry

  • L

• • Structure

  • Alpha helix,random coil

• • Structure Description

  • 28% α-helix was determined from the circular dichroism spectra recorded in 50% TFE/10mM NH4AC solution,and it adopted a random coil structure in an aqueous environment.

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP29106.

DRAMP29106

• • Function

  • Antibacterial activity against Gram-positive bacteria and Gram-negative bacteria.

• ·Literature 1

• • Title

  • Evaluation of the bioactivity of a mastoparan peptide from wasp venom and of its analogues designed through targeted engineering.

• • Pubmed ID

  • 29904274

• • Reference

  • Int J Biol Sci. 2018 Apr 25;14(6):599-607.

• • Author

  • Chen X, Zhang L, Wu Y, Wang L, Ma C, Xi X, Bininda-Emonds ORP, Shaw C, Chen T, Zhou M.