General Information

    • DRAMP ID

    • DRAMP29124

    • Peptide Name

    • Mastoparan-C [A5,8K]

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • N/A

    • Sequence

    • LNLKKLLKVAKKIL

    • Sequence Length

    • 14

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial,Anti-Gram+, Anti-Gram-

    • Target Organism

      • [Ref.33285267]Gram-positive bacteria:Staphylococcus aureus ATCC 25923(MIC=16 µM); Bacillus subtilis ATCC 23857(MIC=4 µM);
      • Gram-negative bacteria:Escherichia coli ATCC 25922(MIC=8 µM); Pseudomonas aeruginosa ATCC 9027(MIC=4 µM); Klebsiella pneumoniae ATCC 700603(MIC=8 µM).

    • Hemolytic Activity

      • [Ref.33285267]15% hemolysis against mouse erythrocytes at 256 µM.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • liposomes

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix,random coil

    • Structure Description

    • The CD spectra in water adopts a random-coil conformation,3.56% α-helix was determined in 50% TFE.

    • Helical Wheel Diagram

    • DRAMP29124 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29124.

Physicochemical Information

    • Formula

    • C78H148N20O16

    • Absent Amino Acids

    • CDEFGHMPQRSTWY

    • Common Amino Acids

    • KL

    • Mass

    • 1622.16

    • PI

    • 10.6

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 8

    • Net Charge

    • +5

    • Boman Index

    • 98

    • Hydrophobicity

    • 0.464

    • Aliphatic Index

    • 195

    • Half Life

      • Mammalian:5.5 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 1

DRAMP29124

Comments Information

    • Function

    • when combined with gentamicin, rifampin, and polymyxin B, it demonstrated synergistic or additive activity against E. coli ATCC 25922 and P. aeruginosa ATCC 9027.

Literature Information

  • ·Literature 1

    • Title

    • Newly designed antimicrobial peptides with potent bioactivity and enhanced cell selectivity prevent and reverse rifampin resistance in Gram-negative bacteria.

    • Reference

    • Eur J Pharm Sci. 2021 Mar 1;158:105665.

    • Author

    • Zhu N, Zhong C, Liu T, Zhu Y, Gou S, Bao H, Yao J, Ni J.