General Information

    • DRAMP ID

    • DRAMP29175

    • Peptide Name

    • 2019-nCoV-HR2P(SARS-CoV-2-S(1168-1203),SARS-HR2P)

    • Source

    • Synthetic construct

    • Family

    • Belongs to the betacoronaviruses spike protein family.

    • Gene

    • S

    • Sequence

    • DISGINASVVNIQKEIDRLNEVAKNLNESLIDLQEL

    • Sequence Length

    • 36

    • Protein Existence

    • Hemology

Activity Information

    • Biological Activity

    • Antimicrobial, Antiviral(SARS-CoV-2)

    • Target Organism

      • [Ref.32047258]Virus:
      • SARS-CoV-2:ihibition of cell-cell fusion in Huh-7 cells(IC50=0.18 µM),inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50=0.98 µM).
      • [Ref.30989115]SARS-CoV:ihibition of cell-cell fusion in Huh-7 cells(IC50=0.52±0.11 µM),inhibition of Pseudovirus (PsV) infection in 293T/ACE2 cells(IC50=2.81 µM);
      • MERS-CoV:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
      • HCoV-OC43:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
      • HCoV-229E:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
      • HCoV-NL63:ihibition of cell-cell fusion in Huh-7 cells(IC50>5 µM);
      • CoV-WIV1:ihibition of cell-cell fusion in 293T/ACE2 cells(IC50=2.73 µM);
      • CoV-Rs3367:ihibition of cell-cell fusion in 293T/ACE2 cells(IC50=3.05 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • liposomes

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29175 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29175.

Physicochemical Information

    • Formula

    • C172H292N48O61

    • Absent Amino Acids

    • CFHMPTWY

    • Common Amino Acids

    • ILN

    • Mass

    • 4008.5

    • PI

    • 4.2

    • Basic Residues

    • 3

    • Acidic Residues

    • 7

    • Hydrophobic Residues

    • 15

    • Net Charge

    • -4

    • Boman Index

    • -6802

    • Hydrophobicity

    • -0.178

    • Aliphatic Index

    • 138.06

    • Half Life

      • Mammalian:1.1 hour
      • Yeast:3 min
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 9

DRAMP29175

DRAMP29175 chydropathy plot

Comments Information

    • Mechanism of action

    • 2019-nCoV HR1 and HR2 regions are able to interact with each other to form 6-HB and suggest that 2019-nCoV-HR2P may inhibit 2019-nCoV fusion with and entry into the target cell.

Literature Information

  • ·Literature 1

    • Title

    • A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike.

    • Reference

    • Sci Adv. 2019 Apr 10;5(4):eaav4580.

    • Author

    • Xia S, Yan L, Xu W, Agrawal AS, Algaissi A, Tseng CK, Wang Q, Du L, Tan W, Wilson IA, Jiang S, Yang B, Lu L.
  • ·Literature 2

    • Title

    • Fusion mechanism of 2019-nCoV and fusion inhibitors targeting HR1 domain in spike protein.

    • Reference

    • Cell Mol Immunol. 2020 Jul;17(7):765-767.

    • Author

    • Xia S, Zhu Y, Liu M, Lan Q, Xu W, Wu Y, Ying T, Liu S, Shi Z, Jiang S, Lu L.