General Information

    • DRAMP ID

    • DRAMP29199

    • Peptide Name

    • MERS-CoV-HR2P-GSGSGC

    • Source

    • Synthetic construct

    • Family

    • Belongs to the betacoronaviruses spike protein family.

    • Gene

    • S

    • Sequence

    • SLTQINTTLLDLTYEMLSLQQVVKALNESYIDLKELGSGSGC

    • Sequence Length

    • 42

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antiviral(SARS-CoV-2)

    • Target Organism

      • [Ref.33082259]Virus:
      • SARS-CoV-2:ihibition of cell-cell fusion in 293T cells(IC50>650 nM,IC90>1000 nM),inhibition of infection in Vero E6 cells(IC50~ 36 nM);
      • SARS-CoV-2_D614G:ihibition of cell-cell fusion in 293T cells(IC50=1000 nM,IC90>1000 nM);
      • SARS-CoV-2_S943P:ihibition of cell-cell fusion in 293T cells(IC50>1000,IC90>1000 nM);
      • SARS-CoV-2_S247R:ihibition of cell-cell fusion in 293T cells(IC50>700 nM,IC90>1000 nM);
      • MERS-CoV:ihibition of cell-cell fusion in 293T cells(IC50=417±180 nM,IC90>1000 nM),inhibition of infection in Vero E6 cells(IC50~ 4 nM);
      • SARS-CoV-1:ihibition of cell-cell fusion in 293T cells(IC50=40±34 nM,IC90>700 nM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • [Ref.33082259]Human embryonic kidney HEK293T cells:<10% Cytotoxicity at 10 µM;Vero E6 cells:12% Cytotoxicity at 10 µM.

    • Binding Target

    • liposomes

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29199 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29199.

Physicochemical Information

    • Formula

    • C200H331N49O69S2

    • Absent Amino Acids

    • FHPRW

    • Common Amino Acids

    • L

    • Mass

    • 4590.23

    • PI

    • 4.18

    • Basic Residues

    • 2

    • Acidic Residues

    • 5

    • Hydrophobic Residues

    • 14

    • Net Charge

    • -3

    • Boman Index

    • -3597

    • Hydrophobicity

    • 0.105

    • Aliphatic Index

    • 118.33

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 2980

    • Absorbance 280nm

    • 72.68

    • Polar Residues

    • 17

DRAMP29199

DRAMP29199 chydropathy plot

Comments Information

    • Mechanism of action

    • The lipopeptide is derived from the C-terminal heptad repeat (HRC) domain of SARS-CoV-2 S that potently inhibits infection by SARS-CoV-2.

Literature Information

  • ·Literature 1

    • Title

    • Inhibition of Coronavirus Entry In Vitro and Ex Vivo by a Lipid-Conjugated Peptide Derived from the SARS-CoV-2 Spike Glycoprotein HRC Domain.

    • Reference

    • mBio. 2020 Oct 20;11(5):e01935-20.

    • Author

    • Outlaw VK, Bovier FT, Mears MC, Cajimat MN, Zhu Y, Lin MJ, Addetia A, Lieberman NAP, Peddu V, Xie X, Shi PY, Greninger AL, Gellman SH, Bente DA, Moscona A, Porotto M.