General Information

    • DRAMP ID

    • DRAMP29203

    • Peptide Name

    • CMK

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • RVKR

    • Sequence Length

    • 4

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antiviral(SARS-CoV-2)

    • Target Organism

      • [Ref.33007239]Virus:SARS-CoV-2:inhibition of virus production in Vero E6 cells(IC50=0.057 µM).
      • [Ref.31683742]Virus:Zika virus (ZIKV):inhibition of virus release in Vero cells(IC50=18.59 µM);Japanese encephalitis virus (JEV):inhibition of virus release in BHK-21 cells(IC50=19.91 µM).
      • [Ref.23617302]Virus:Hepatitis B virus (HBV):Inhibition of HBeAg secretion in HepG2.2.15 cells(26±11% inhibition at 20 µM,21±13% Inhibition at 100 µM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • [Ref.31683742]Vero cells:CC50=712.9 µM.[Ref.33007239]Vero E6 cells:IC50=318.2 µM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Decanoyl(C10)

    • C-terminal Modification

    • chloromethylketone(CMK)

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29203 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29203.

Physicochemical Information

    • Formula

    • C23H47N11O5

    • Absent Amino Acids

    • ACDEFGHILMNPQSTWY

    • Common Amino Acids

    • R

    • Mass

    • 557.7

    • PI

    • 12.01

    • Basic Residues

    • 3

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 1

    • Net Charge

    • +3

    • Boman Index

    • -3135

    • Hydrophobicity

    • -2.175

    • Aliphatic Index

    • 72.5

    • Half Life

      • Mammalian:1 hour
      • Yeast:2 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 0

    • Absorbance 280nm

    • 0

    • Polar Residues

    • 0

DRAMP29203

Comments Information

    • Mechanism of action

    • CMK blocks virus entry, but it further suppresses cleavage of spikes and the syncytium.

Literature Information

  • ·Literature 1

    • Title

    • Therapeutic potential of furin inhibitors for the chronic infection of hepatitis B virus.

    • Reference

    • Liver Int. 2013 Sep;33(8):1230-8.

    • Author

    • Pang YJ, Tan XJ, Li DM, Zheng ZH, Lei RX, Peng XM.
  • ·Literature 2

    • Title

    • Decanoyl-Arg-Val-Lys-Arg-Chloromethylketone: An Antiviral Compound That Acts against Flaviviruses through the Inhibition of Furin-Mediated prM Cleavage.

    • Reference

    • Viruses. 2019 Oct 31;11(11):1011.

    • Author

    • Imran M, Saleemi MK, Chen Z, Wang X, Zhou D, Li Y, Zhao Z, Zheng B, Li Q, Cao S, Ye J.
  • ·Literature 3

    • Title

    • Furin Inhibitors Block SARS-CoV-2 Spike Protein Cleavage to Suppress Virus Production and Cytopathic Effects.

    • Reference

    • Cell Rep. 2020 Oct 13;33(2):108254.

    • Author

    • Cheng YW, Chao TL, Li CL, Chiu MF, Kao HC, Wang SH, Pang YH, Lin CH, Tsai YM, Lee WH, Tao MH, Ho TC, Wu PY, Jang LT, Chen PJ, Chang SY, Yeh SH.