General Information

    • DRAMP ID

    • DRAMP29222

    • Peptide Name

    • IBP27

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • SVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIK

    • Sequence Length

    • 37

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antiviral(SARS-CoV-2)

    • Target Organism

      • [Ref.34057039]Virus:
      • SARS-CoV-2:inhibition of SARS-CoV-2 S protein-mediated cell-cell fusion(IC50=0.32 nM);inhibition of pseudovirus infections in 293T/ACE2 cells(IC50=2.77 nM);inhibition of pseudovirus infections in Huh-7 cells(IC50=1.54 nM),inhibition of live SARS-CoV-2 infection in Vero cells(IC50=29.85 nM);
      • SARS-CoV-2 D614G:inhibition of pseudovirus infections in Huh-7 cells(IC50=6.75 nM);
      • SARS-CoV-2 N501Y:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.87 nM);
      • SARS-CoV-2 ΔH69-V70:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.56 nM);
      • SARS-CoV-2 E484K:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.55 nM);
      • SARS-CoV-2 B.1.1.7:inhibition of pseudovirus infections in Huh-7 cells(IC50=7.18 nM);
      • SARS-CoV-2 B.1.351:inhibition of pseudovirus infections in Huh-7 cells(IC50=8.25 nM);
      • SARS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=24.95 nM);
      • MERS-CoV:inhibition of pseudovirus infections in Huh-7 cells(IC50=60.08 nM);
      • HCoV-NL63:inhibition of pseudovirus infections in Huh-7 cells(IC50=179.53 nM);
      • HCoV-229E:inhibition of pseudovirus infections in Huh-7 cells(IC50=231.18 nM).

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

      • [Ref.34057039]Huh-7 cells:CC50=4.04 μM;Vero-E6 cells:CC50=5.05 μM.

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • PEG8-K(Chol)

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • 12% α-helicity in phosphate-buffered saline (PBS; pH 7.2) with a final concentration of 10 μM and incubated at 37°C

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP29222 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29222.

Physicochemical Information

    • Formula

    • C192H317N51O63

    • Absent Amino Acids

    • CFHMPTW

    • Common Amino Acids

    • EL

    • Mass

    • 4347.93

    • PI

    • 4.77

    • Basic Residues

    • 5

    • Acidic Residues

    • 7

    • Hydrophobic Residues

    • 13

    • Net Charge

    • -2

    • Boman Index

    • -7972

    • Hydrophobicity

    • -0.603

    • Aliphatic Index

    • 121.08

    • Half Life

      • Mammalian:1.9 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 2980

    • Absorbance 280nm

    • 82.78

    • Polar Residues

    • 9

DRAMP29222

DRAMP29222 chydropathy plot

Comments Information

    • Mechanism of action

    • Peptides derived from HR2 sequences of viral fusion proteins, including the S protein of emerging CoVs, can competitively bind to the HR1 domain and block the formation of the viral 6-HB core, thereby inhibiting infection of the virus from which they were derived.

Literature Information

  • ·Literature 1

    • Title

    • Structure-based design and characterization of novel fusion-inhibitory lipopeptides against SARS-CoV-2 and emerging variants.

    • Reference

    • Emerg Microbes Infect. 2021 Dec;10(1):1227-1240.

    • Author

    • Yu D, Zhu Y, Jiao T, Wu T, Xiao X, Qin B, Chong H, Lei X, Ren L, Cui S, Wang J, He Y.