General Information

    • DRAMP ID

    • DRAMP21493

    • Peptide Name

    • LL-IIIs-6b

    • Sequence

    • VNⓍKKIⓍPKⓍIKVⓍK

    • Sequence Length

    • 15

    • Original Sequence

    • VNWKKILGKIIKVVK

    • Source

    • Synthetic construct

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Comments

    • Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.

    • Target Organism

      • [Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.9 μM), Bacillus subtilis (MIC = 0.8 μM), Staphylococcus aureus (MIC = 50 μM);
      • Gram-negative bacteria: E.coli (MIC = 7.8 μM), Pseudomonas aeruginosa (MIC = 63 μM);
      • Fungi: Candida albicans (MIC > 100 μM).

    • Hemolytic Activity

      • [Ref.22526241] LC50 = 82μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

Structure Information

    • Linear/Cyclic

    • Cyclic (Stapled)

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Special Amino Acid and Stapling Position

    • ①The Ⓧ (position: 3, 7, 10 and 14) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (3) and Ⓧ (7), Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling respectively.

    • Stereochemistry

    • L, trans(around the S₅-Pro8 peptide bond)

    • Secondary Structure

    • ①33% α-helical content in water. ②38% α-helical content in 50% TFE. ③35% α-helical content in 8mM SDS.

    • Structure Description

    • No other descriptive information about the structure found in the literature

    • Helical Wheel Diagram

    • DRAMP21493 helical wheel diagram

    • Predicted Structure

Literature Information

  • Literature 1

    • Title

    • Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera

    • Reference

    • Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 25.

    • Author

    • Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský