General Information

    • DRAMP ID

    • DRAMP29009

    • Peptide Name

    • A4K14-citropin1.1-Sp3

    • Sequence

    • GLFAVⓍKKVⓍSVIKGL

    • Sequence Length

    • 16

    • Original Sequence

    • GLFAVIKKVASVIKGL

    • Source

    • Synthetic construct

Activity Information

    • Biological Activity

    • Antimicrobial, Anticancer

    • Comments

    • Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.

    • Target Organism

      • [Ref.33363118] Cancer cell lines: C4-2B (IC50 = 17.89 μM), A549 (IC50 = 12.11 μM), U87 (IC50 = 11.93 μM), MCF-7 (11.92 μM)

    • Hemolytic Activity

      • No hemolytic activity information found.

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

Structure Information

    • Linear/Cyclic

    • Cyclic (Stapled)

    • N-terminal Modification

    • Acetylation

    • C-terminal Modification

    • Amidation

    • Special Amino Acid and Stapling Position

    • ① The Ⓧ (position: 6 and 10) in sequence indicate (S)-2-(4-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.

    • Stereochemistry

    • L

    • Secondary Structure

    • Helicity = 49.2% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)

    • Structure Description

    • [Ref.33363118] CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.

    • Helical Wheel Diagram

    • DRAMP29009 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP29009.

Literature Information

  • Literature 1

    • Title

    • Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides

    • Reference

    • Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.

    • Author

    • Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu