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Peptide Name
- peptide 9 (derived from OH-CM6)
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Sequence
- KFFKKLKKAVKⓀGFKKFAⓀV
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Original Sequence
- KFFKKLKKAVKKGFKKFAKV
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Source
- Synthetic construct
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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- Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.
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Target Organism
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- [Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 8 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);
- Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 16 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 16 μg/mL)
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Hemolytic Activity
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- [Ref.32216308] It has 5.4% hemolysis against red blood cells at peptide concentration of 320 μg/mL
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Cytotoxicity
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No cytotoxicity information found in the reference(s) presented
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Linear/Cyclic
- Cyclic (Stapled)
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Special Amino Acid and Stapling Position
- ①The Ⓚ (position: 12 and 19) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (19) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.
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Secondary Structure
- Random coils in PBS.
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Structure Description
- ①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.
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There is no predicted structure for DRAMP21475.
- Literature 1
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Title
- Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides
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Reference
- J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.
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Author
- Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang