General Information
-
DRAMP ID
- DRAMP21621
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Peptide Name
- sKFGF
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Sequence
- AAⓀLLPⒹLLAAP
-
Sequence Length
- 12
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Original Sequence
- AAVLLPVLLAAP
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Source
- Synthetic construct
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Comments
- Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.
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Target Organism
-
- [Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC > 256 μg/mL), Enterococcus faecalis (MIC > 256 μg/mL);
- Gram-negative bacteria: Escherichia coli (MIC > 256 μg/mL), Pseudomonas aeruginosa (MIC > 256 μg/mL)
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Hemolytic Activity
-
- [Ref.28921993] It has 0.4%, 0%, 0.1%, 0.2%, 0.1%, 0.7%, 1.8% and 5.6% hemolysis against human red blood cells at 5, 7.5, 10, 15, 20, 25, 30 and 40 μg/ml.
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Cytotoxicity
-
- [Ref.28921993] The toxicity of sKFGF toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM.
Structure Information
-
Linear/Cyclic
- Cyclic (Stapled)
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Special Amino Acid and Stapling Position
- Ⓚ (3) and Ⓓ (7) are cross-linked by lactam stapling through the polar amide bond of a lactam bridge.
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Stereochemistry
- L
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Secondary Structure
- ①Disordered (or unstructured) conformation in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②Around 22% average α-helix content in various membranes environments [13% in POPC; 32% in
-
Structure Description
- In the cases of sDRIM and sKFGF, stapling did not induce any conformational change, as these analogues remained just as unstructured.
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Helical Wheel Diagram
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Predicted Structure
- There is no predicted structure for DRAMP21621.
Physicochemical Information
-
Formula
- C₅₆H₉₆N₁₂O₁₂
Absent Amino Acids
- CEFGHIMNQRSTVWY
Common Amino Acids
- AL
Mass
- 1129.46
PI
- /
-
Basic Residues
- 1
Acidic Residues
- 1
Hydrophobic Residues
- 8
Hydrophobicity
- /
Polar Residues
- 0
DRAMP21621
Literature Information
- Literature 1
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Title
- Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties.
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Pubmed ID
- 28921993
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Reference
- J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26.
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Author
- Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich