General Information
-
DRAMP ID
- DRAMP28989
-
Peptide Name
- peptide 5 (Derived from Mag2)
-
Sequence
- GIKKFLKSⓍKKFⓍKAFK
-
Sequence Length
- 17
-
Original Sequence
- GIGKFLHSAKKFGKAFVGEIMNS
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Source
- Synthetic construct
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Comments
- Function: Antibacterial activity against Gram-positive and Gram-negative bacteria
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Target Organism
-
- [Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 12.5 μM).
- Gram-negative bacteria: Escherichia coli (MIC = 1.56 μM), Pseudomonas aeruginosa (MIC = 6.25 μM), multiple-drug resistant P.aeruginosa (MIC = 3.125 μM)
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Hemolytic Activity
-
- [Ref.33466998] It has hemolysis against human red blood cells at 1.56 μM
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
Structure Information
-
Linear/Cyclic
- Cyclic (Stapled)
-
N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Special Amino Acid and Stapling Position
- ①The Ⓧ (position: 9 and 13) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.
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Stereochemistry
- L
-
Secondary Structure
- α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM
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Structure Description
- [Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.
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Helical Wheel Diagram
-
Predicted Structure
- There is no predicted structure for DRAMP28989.
Physicochemical Information
-
Formula
- C₁₀₃H₁₇₁N₂₅O₁₈
Absent Amino Acids
- CDEHMNPQRTVWY
Common Amino Acids
- K
Mass
- 2047.61
PI
- /
-
Basic Residues
- 7
Acidic Residues
- 0
Hydrophobic Residues
- 6
Hydrophobicity
- /
Polar Residues
- 2
DRAMP28989
Literature Information
- Literature 1
-
Title
- Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence
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Pubmed ID
- 33466998
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Reference
- Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.
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Author
- Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu