• DRAMP ID

    • DRAMP28992
    • Peptide Name

    • peptide 8 (Derived from Mag2)
    • Sequence

    • GIKKFLKⓏAKKFVKⓍFK
    • Sequence Length

    • 17
    • Original Sequence

    • GIGKFLHSAKKFGKAFVGEIMNS
    • Source

    • Synthetic construct
    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-
    • Comments

    • Function: Antibacterial activity against Gram-positive and Gram-negative bacteria
    • Target Organism

      • [Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC > 50 μM).
      • Gram-negative bacteria: Escherichia coli (MIC = 6.25 μM), Pseudomonas aeruginosa (MIC > 50 μM), multiple-drug resistant P.aeruginosa (MIC = 3.125 μM)
    • Hemolytic Activity

      • [Ref.33466998] It has hemolysis against human red blood cells at 0.19 μM
    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented
    • Linear/Cyclic

    • Cyclic (Stapled)
    • N-terminal Modification

    • Free
    • C-terminal Modification

    • Amidation
    • Special Amino Acid and Stapling Position

    • ①The Ⓩ (position: 8) in sequence indicates (R)-7-octenyl alanine. ②The Ⓧ (position: 15) in sequence indicates (S)-4-pentenyl alanine. ③ Ⓩ (8) and Ⓧ (15) are cross-linked by hydrocarbon stapling through a undec-4-enyl staple.
    • Stereochemistry

    • L
    • Secondary Structure

    • α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM
    • Structure Description

    • [Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.
    • Helical Wheel Diagram

    • DRAMP28992 helical wheel diagram
    • Predicted Structure

    • There is no predicted structure for DRAMP28992.
    • Formula

    • C₁₀₈H₁₈₁N₂₅O₁₇
    • Absent Amino Acids

    • CDEHMNPQRSTWY
    • Common Amino Acids

    • K
    • Mass

    • 2101.74
    • PI

    • /
    • Basic Residues

    • 7
    • Acidic Residues

    • 0
    • Hydrophobic Residues

    • 7
    • Hydrophobicity

    • /
    • Polar Residues

    • 1

DRAMP28992

  • Literature 1
    • Title

    • Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence
    • Reference

    • Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.
    • Author

    • Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu