General Information
-
DRAMP ID
- DRAMP28995
-
Peptide Name
- peptide C12-2 (Derived from Mag2)
-
Sequence
- ⓍIKKⓍLKSAKKFVKAFK
-
Sequence Length
- 17
-
Original Sequence
- GIGKFLHSAKKFGKAFVGEIMNS
-
Source
- Synthetic construct
Activity Information
-
Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-
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Comments
- Function: Antibacterial activity against Gram-positive and Gram-negative bacteria
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Target Organism
-
- [Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 6.25 μM).
- Gram-negative bacteria: Escherichia coli (MIC = 6.25 μM), Pseudomonas aeruginosa (MIC = 12.5 μM), multiple-drug resistant P.aeruginosa (MIC = 25 μM)
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Hemolytic Activity
-
- [Ref.33466998] It has hemolysis against human red blood cells at 0.78 μM
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
Structure Information
-
Linear/Cyclic
- Cyclic (Stapled)
-
N-terminal Modification
- Laurylation
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C-terminal Modification
- Amidation
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Special Amino Acid and Stapling Position
- ①The Ⓧ (position: 1 and 5) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (1) and Ⓧ (5) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.
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Stereochemistry
- L
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Secondary Structure
- α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM
-
Structure Description
- [Ref.33466998] Peptide C6-2 and C12-2 showed a curve similar to that of peptide 2
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Helical Wheel Diagram
-
Predicted Structure
- There is no predicted structure for DRAMP28995.
Physicochemical Information
-
Formula
- C₁₁₂H₁₉₅N₂₅O₁₉
Absent Amino Acids
- CDEGHMNPQRTWY
Common Amino Acids
- K
Mass
- 2195.9
PI
- /
-
Basic Residues
- 7
Acidic Residues
- 0
Hydrophobic Residues
- 7
Hydrophobicity
- /
Polar Residues
- 1
DRAMP28995
Literature Information
- Literature 1
-
Title
- Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence
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Pubmed ID
- 33466998
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Reference
- Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.
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Author
- Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu