General Information
-
DRAMP ID
- DRAMP29239
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Peptide Name
- hACE2(21-55)A36K-F40E
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Sequence
- IEEQAKTFLDKFNHEⓀEDLⒺYQSSLASWNYNTNIT
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Sequence Length
- 35
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Original Sequence
- IEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNIT
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Source
- Synthetic construct
Activity Information
-
Biological Activity
- Antimicrobial, Antiviral(SARS-CoV-2)
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Comments
- Mechanism of action:The stapled peptide inhibit the RBD-hACE2 complex formation, and hACE2 α1-helix-based peptidomimetics could potentially prevent SARS-CoV-2 from entering the human cells through hACE2 and thus inhibit subsequent viral replication.
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Target Organism
-
- [Ref.33651072]Virus:SARS-CoV-2:inhibition of SARS-CoV-2 Spike protein-hACE2 complex formation(IC50=3.6 μM).
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Hemolytic Activity
-
- No hemolytic activity information found.
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Cytotoxicity
- No cytotoxicity information found in the reference(s) presented
Structure Information
-
Linear/Cyclic
- Cyclic (Stapled)
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N-terminal Modification
- Free
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C-terminal Modification
- Free
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Special Amino Acid and Stapling Position
- Ⓚ (16) and Ⓔ (20) are corss-linked by lactam stapling.
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Stereochemistry
- L
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Secondary Structure
- ①52% α-helical content in 10 mM PBS at pH 7.4 with 30% TFE at 298 K.②6-13% α-helical content in 10 mM PBS at pH 7.4 and 298 K.
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Structure Description
- Low helicity for stapled hACE2 peptides in absence of TFE, with predictors of helicity averaging to 6-13% helical content. In the presence of TFE, α-helical structures can be observed for various synthetic hACE2 peptides with predictors averaging from 11 to 52% helical content
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Helical Wheel Diagram
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Predicted Structure
- There is no predicted structure for DRAMP29239.
Physicochemical Information
-
Formula
- C188H272N46O62
Absent Amino Acids
- CGMPRV
Common Amino Acids
- EN
Mass
- 4168.5
PI
- 4.35
-
Basic Residues
- 3
Acidic Residues
- 6
Hydrophobic Residues
- 12
Hydrophobicity
- -76.57
Polar Residues
- 12
DRAMP29239
Literature Information
- Literature 1
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Title
- Targeting SARS-CoV-2 spike protein by stapled hACE2 peptides.
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Pubmed ID
- 33651072
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Reference
- Chem Commun (Camb). 2021 Apr 4;57(26):3283-3286.
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Author
- Maas MN , Hintzen JCJ , Löffler PMG , Mecinović J .