General Information

    • DRAMP ID

    • DRAMP29335

    • Peptide Name

    • LS-Ascaphin-8 P1

    • Sequence

    • GFKDLLKGAAⓀALVⓀTVLF

    • Sequence Length

    • 19

    • Original Sequence

    • GFKDLLKGAAKALVKTVLF

    • Source

    • Synthetic construct

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Comments

    • Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.

    • Target Organism

      • [Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=4 μg/mL), Staphylococcus aureus BNCC 186335(MIC=2 μg/mL), Staphylococcus aureus(MRSA)(MIC=8 μg/mL) ;
      • Gram-negative bacteria:P. aeruginosa(MIC=128 μg/mL), E. coli(MIC=8 μg/mL), Cephalosporin-resistant E. coli(MIC=16 μg/mL).

    • Hemolytic Activity

      • [Ref.34968054]MHC=32 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.

    • Cytotoxicity

      • [Ref.34968054]Human embryonic kidney HEK293T cells: LC90=128 µg/ml.

Structure Information

    • Linear/Cyclic

    • Cyclic (Stapled)

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Special Amino Acid and Stapling Position

    • ①The Ⓚ (position: 11 and 15) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (11) and Ⓚ (15) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.

    • Stereochemistry

    • L

    • Secondary Structure

    • No specific results about the strcture presented in the forms of tables, graphs or words

    • Structure Description

    • No other descriptive information about the structure found in the literature

    • Helical Wheel Diagram

    • DRAMP29335 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP29335.

Physicochemical Information

    • Formula

    • C103H161N27O16

    • Absent Amino Acids

    • ACDEGHILMNPQSTWY

    • Common Amino Acids

    • K

    • Mass

    • 2019.5

    • PI

    • 10

    • Basic Residues

    • 8

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 7

    • Hydrophobicity

    • -66.67

    • Polar Residues

    • 0

DRAMP29335

Literature Information

  • Literature 1

    • Title

    • Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.

    • Reference

    • J Med Chem. 2022 Jan 13;65(1):579-591. 

    • Author

    • Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J.