General Information

    • DRAMP ID

    • DRAMP29342

    • Peptide Name

    • LS-Cathelicidin-BF-15-a1-2

    • Sequence

    • VⓀRFKⓀFFRKFKKFV

    • Sequence Length

    • 15

    • Original Sequence

    • VKRFKKFFRKFKKFV

    • Source

    • Synthetic construct

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-

    • Comments

    • Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.

    • Target Organism

      • [Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=16μg/mL), Staphylococcus aureus BNCC 186335(MIC=2 μg/mL), Staphylococcus aureus(MRSA)(MIC=4 μg/mL) ;
      • Gram-negative bacteria:P. aeruginosa(MIC=8 μg/mL), E. coli(MIC=4 μg/mL), Cephalosporin-resistant E. coli(MIC=16 μg/mL).

    • Hemolytic Activity

      • [Ref.34968054]MHC>128 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.

    • Cytotoxicity

      • [Ref.34968054]Human embryonic kidney HEK293T cells: LC50=100 µg/ml.

Structure Information

    • Linear/Cyclic

    • Cyclic (Stapled)

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Special Amino Acid and Stapling Position

    • ①The Ⓚ (position: 2 and 6) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (2) and Ⓚ (6) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.

    • Stereochemistry

    • L

    • Secondary Structure

    • No specific results about the strcture presented in the forms of tables, graphs or words

    • Structure Description

    • No other descriptive information about the structure found in the literature

    • Helical Wheel Diagram

    • DRAMP29342 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP29342.

Physicochemical Information

    • Formula

    • C139H263N41O27

    • Absent Amino Acids

    • CDEHMNPQRTVWY

    • Common Amino Acids

    • K

    • Mass

    • 2033.58

    • PI

    • 12.05

    • Basic Residues

    • 16

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 6

    • Hydrophobicity

    • -168.4

    • Polar Residues

    • 3

DRAMP29342

Literature Information

  • Literature 1

    • Title

    • Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.

    • Reference

    • J Med Chem. 2022 Jan 13;65(1):579-591. 

    • Author

    • Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J.