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Original Sequence
- LARRLAR
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Source
- Synthetic construct
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Biological Activity
- Antimicrobial, Antibacterial, Anti-Gram-
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- Function: Antibacterial activity against Gram-negative bacteria.
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Target Organism
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- [Ref.37531494]Gram-negativebacteria:E. coli ATCC25922(MIC=8.00 μM), E. coli UB1005(MIC=4.00 μM), E. coli K99(MIC=8.00 μM), E. coli 987P(MIC= 4.00 μM), S. typhimurium ATCC14028(MIC=32.00 μM), P. aeruginosa PAO1(MIC=16.00 μM).
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Hemolytic Activity
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- [Ref.37531494]MHC>256 μM. MHC is the minimum peptide concentration that caused >5% hemolysis of hRBCs.
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Cytotoxicity
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- [Ref.37531494]<10% cytotoxicity against human embryonic kidney cells (HEK293T) and intestinal porcine enterocyte cells (IPEC-J2) up to 128 μM. 71.50% cell viability against murine macrophage cells (RAW264.7) at 128 μM.
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Linear/Cyclic
- Cyclic (Stapled)
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N-terminal Modification
- Free
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C-terminal Modification
- Amidation
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Special Amino Acid and Stapling Position
- ①The Ⓧ (position: 2 and 6) in sequence indicates S-(2,4)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling respectively.
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Secondary Structure
- α-helical conformation in an aqueous environment(phosphate buffered saline, PBS), negative electric environment(sodium dodecyl sulfate, SDS), and hydrophobic environment(tri-fluoroethyl alcohol, TFE).
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Structure Description
- No other descriptive information about the structure found in the literature
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There is no predicted structure for DRAMP29360.
- Literature 1
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Title
- Ultrashort All-Hydrocarbon Stapled α-Helix Amphiphile as a Potent and Stable Antimicrobial Compound.
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Reference
- J Med Chem. 2023 Aug 24;66(16):11414-11427.
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Author
- Shao C, Jian Q, Li B, Zhu Y, Yu W, Li Z, Shan A.