General Information

    • DRAMP ID

    • DRAMP29360

    • Peptide Name

    • stRRL

    • Sequence

    • LⓧRRLⓧR

    • Sequence Length

    • 7

    • Original Sequence

    • LARRLAR

    • Source

    • Synthetic construct

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Comments

    • Function: Antibacterial activity against Gram-negative bacteria.

    • Target Organism

      • [Ref.37531494]Gram-negativebacteria:E. coli ATCC25922(MIC=8.00 μM), E. coli UB1005(MIC=4.00 μM), E. coli K99(MIC=8.00 μM), E. coli 987P(MIC= 4.00 μM), S. typhimurium ATCC14028(MIC=32.00 μM), P. aeruginosa PAO1(MIC=16.00 μM).

    • Hemolytic Activity

      • [Ref.37531494]MHC>256 μM. MHC is the minimum peptide concentration that caused >5% hemolysis of hRBCs.

    • Cytotoxicity

      • [Ref.37531494]<10% cytotoxicity against human embryonic kidney cells (HEK293T) and intestinal porcine enterocyte cells (IPEC-J2) up to 128 μM. 71.50% cell viability against murine macrophage cells (RAW264.7) at 128 μM.

Structure Information

    • Linear/Cyclic

    • Cyclic (Stapled)

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Amidation

    • Special Amino Acid and Stapling Position

    • ①The Ⓧ (position: 2 and 6) in sequence indicates S-(2,4)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling respectively.

    • Stereochemistry

    • L

    • Secondary Structure

    • α-helical conformation in an aqueous environment(phosphate buffered saline, PBS), negative electric environment(sodium dodecyl sulfate, SDS), and hydrophobic environment(tri-fluoroethyl alcohol, TFE).

    • Structure Description

    • No other descriptive information about the structure found in the literature

    • Helical Wheel Diagram

    • DRAMP29360 helical wheel diagram

    • Predicted Structure

    • There is no predicted structure for DRAMP29360.

Physicochemical Information

    • Formula

    • C101H179N27O21

    • Absent Amino Acids

    • CDEHMQSTVWY

    • Common Amino Acids

    • KL

    • Mass

    • 935.56

    • PI

    • 12.3

    • Basic Residues

    • 5

    • Acidic Residues

    • 0

    • Hydrophobic Residues

    • 11

    • Hydrophobicity

    • 59.47

    • Polar Residues

    • 2

DRAMP29360

Literature Information

  • Literature 1

    • Title

    • Ultrashort All-Hydrocarbon Stapled α-Helix Amphiphile as a Potent and Stable Antimicrobial Compound. 

    • Reference

    • J Med Chem. 2023 Aug 24;66(16):11414-11427. 

    • Author

    • Shao C, Jian Q, Li B, Zhu Y, Yu W, Li Z, Shan A.