General Information

    • DRAMP ID

    • DRAMP29936

    • Peptide Name

    • Api796

    • Source

    • Synthetic construct

    • Family

    • Not found

    • Gene

    • Not found

    • Sequence

    • guOIOIOIORPVYOPRPRPPHPRL

    • Sequence Length

    • 22

    • UniProt Entry

    • No entry found

    • Protein Existence

    • Not found

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram-

    • Target Organism

      • Gram-negative bacterium: Escherichia coli ATCC 25922(MIC=8µg/ml), Klebsiella pneumoniae DSM 681(MIC=16 µg/ml), Pseudomonas aeruginosa DSM 1117 in 50% MHB(MIC=8 µg/ml), Pseudomonas aeruginosa DSM 3227 in 50% MHB(MIC=16 µg/ml), Pseudomonas aeruginosa DSM 9644 in 50% MHB(MIC=16 µg/ml), Pseudomonas aeruginosa DSM 1117 in 100% MHB(MIC=64 µg/ml), Pseudomonas aeruginosa DSM 3227 in 100% MHB(MIC=128 µg/ml), Pseudomonas aeruginosa DSM 9664in 100% MHB(MIC=128 µg/ml).

    • Hemolytic Activity

      • [Ref.27243004] Hemolytic grades of peptide is below 2%, non-hemolytic to human erythrocytes even at high concentrations of 0.6 g/L.

    • Cytotoxicity

      • [Ref.27243004]IC50>0.6 g/L on rat cardiomyocytes, IC50>0.6 g/L on Hek293, IC50>0.6 g/L on HeLa.

    • Binding Target

    • Not found

Stability Information

    • Serum or Protease Type

    • Mouse serum

    • Stability Data

    • Half-life time (mouse serum )=249min

    • Assay

    • RP-HPLC

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • gu = N,N,N′,N′-tetramethylguanidino

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • O = L-ornithine, I=Ile-Orn-motifs

    • Stereochemistry

    • L

    • Structure

    • Not found

    • Structure Description

    • Not found

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP29936.

Literature Information

  • ·Literature 1

    • Title

    • N-Terminal Ile-Orn- and Trp-Orn-Motif Repeats Enhance Membrane Interaction and Increase the Antimicrobial Activity of Apidaecins against Pseudomonas aeruginosa

    • Reference

    • Front Cell Dev Biol. 2016 May 10;4:39

    • Author

    • Bluhm ME, Schneider VA, Schäfer I, Piantavigna S, Goldbach T, Knappe D, Seibel P, Martin LL, Veldhuizen EJ, Hoffmann R.