• • DRAMP ID

  • DRAMP00032

• • Peptide Name

  • Ruminococcin A (RumA; Bacteriocin)

• • Source

  • Ruminococcus gnavus & Ruminococcus hansenii (Gram-positive bacteria)

• • Family

  • Belongs to the type A lantibiotic family (Class I bacteriocin)

• • Gene

  • rumA1 AND rumA2 AND

• • Sequence

  • GNGVLKTISHECNMNTWQFLFTCC

• • Sequence Length

  • 24

• • UniProt Entry

  • P83674,P83676,P83677,Q8VLK0,Q9K381,P83675,P83679,P83680,Q8VLK0

• • Protein Existence

  • Protein level

• • Biological Activity

  • Antimicrobial, Antibacterial, Anti-Gram+

• • Target Organism

  • • Gram-positive bacteria: Bifidobacterium longum (strain: ATCC 15707) (MIC=75 µg/ml), Bacillus cereus (strain: Z4222, TZ415) (MIC=75 µg/ml), Eubacterium contortum (strain: ATCC 25540) (MIC=32.5 µg/ml), Ruminococcus obeum (strain: ATCC 29174) (MIC=32.5 µg/ml), R. gnavus (strain: ATCC 29149) (MIC=75 µg/ml), Clostridium perfringens (strain: CpA) (MIC=75 µg/ml), Clostridium difficile serogroup A (strain: B-1) (MIC=75 µg/ml), C. difficile serogroup C (strain: C253) (MIC=75 µg/ml), C. difficile serogroup S3 (strain: 79685) (MIC=75 µg/ml), C. botulinum type A (strain: CIP 38) (MIC=75 µg/ml), C. botulinum type B (strain: NCTC 7273) (MIC=75 µg/ml), C. botulinum type E (strain: NCTC 8266) (MIC=75 µg/ml), C. bifermentans (strain: NCTC 506) (MIC=75 µg/ml).

• • Hemolytic Activity

  • • No hemolysis information or data found in the reference(s) presented in this entry

• • Cytotoxicity

  • No cytotoxicity information found in the reference(s) presented

• • Binding Target

  • Cell membrane

• • Linear/Cyclic

  • Cyclic (very possibly)

• • N-terminal Modification

  • Free

• • C-terminal Modification

  • Cyclization (possibly)

• • Nonterminal Modifications and Unusual Amino Acids

  • ① It is highly probable that (i) Ser9 and the putative Cys23 and (ii) the putative His22 and Thr24 are associated in a Lanthionine (Lan) and a 3-methyllanthionine (Me-Lan) residue, respectively. ②The residues at position 7 and 16 are (Z)-2,3-didehydrobutyrine (Dhb).

• • Stereochemistry

  • L

• • Structure

  • Not found

• • Structure Description

  • Not found

• • Helical Wheel Diagram

  • 

• • PDB ID

  • None

• Predicted Structure

• There is no predicted structure for DRAMP00032.

DRAMP00032

• • Function

  • Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. Ruminococcin A is a broad spectrum bacteriocin exhibiting activity against a wide range of pathogenic clostridia and B.longum.

• • Developmental stage

  • Highest expression in mid to late logarithmic growth phase, after which expression decreases slowly before leveling off in the stationary growth phase.

• • Induction

  • By trypsin.

• • PTM

  • There are one didehydrobutyrine (Dhb) at T16, one lanthionine (S9-23) and two Beta-methyllanthionines (T7-C12; T22-C24).

• ·Literature 1

• • Title

  • Ruminococcin A, a new lantibiotic produced by a Ruminococcus gnavus strain isolated from human feces.

• • Pubmed ID

  • 11526013

• • Reference

  • Appl Environ Microbiol. 2001 Sep;67(9):4111-4118.

• • Author

  • Dabard J, Bridonneau C, Phillipe C, Anglade P, Molle D, Nardi M, Ladiré M, Girardin H, Marcille F, Gomez A, Fons M.

• ·Literature 2

• • Title

  • Trypsin mediates growth phase-dependent transcriptional tegulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.

• • Pubmed ID

  • 11741840

• • Reference

  • J Bacteriol. 2002 Jan;184(1):18-28.

• • Author

  • Gomez A, Ladiré M, Marcille F, Fons M.

• ·Literature 3

• • Title

  • Distribution of genes encoding the trypsin-dependent lantibiotic ruminococcin A among bacteria isolated from human fecal microbiota.

• • Pubmed ID

  • 12089024

• • Reference

  • Appl Environ Microbiol. 2002 Jul;68(7):3424-3431.

• • Author

  • Marcille F, Gomez A, Joubert P, Ladiré M, Veau G, Clara A, Gavini F, Willems A, Fons M.