General Information

    • DRAMP ID

    • DRAMP00128

    • Peptide Name

    • Plantaricin J (PlnJ; Bacteriocin)

    • Source

    • Lactobacillus plantarum C11 (Gram-positive bacteria)

    • Family

    • Belongs to the class IIb bacteriocin

    • Gene

    • plnJ

    • Sequence

    • GAWKNFWSSLRKGFYDGEAGRAIRR

    • Sequence Length

    • 25

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antibacterial, Anti-Gram+

    • Target Organism

      • Gram-positive bacteria: Pediococcus pentosaceus Pac 1.0 (MIC=50 nM), Lactobacillus plantarum 965 (MIC=0.1 nM), L. sake NCDO 2714 (MIC=50 nM), Leptostylus viridescens NCDO 1655 (MIC=5 nM). [PlnJ:PlnK=1:1]

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Linear

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Free

    • Nonterminal Modifications and Unusual Amino Acids

    • None

    • Stereochemistry

    • L

    • Structure

    • Alpha helix

    • Structure Description

    • In DPC micelles, PlnJ has an N-terminal amphiphilic alpha-helix between Trp-3 and Tyr-15. Crucial residues: Gly-13 in PlnJ is very sensitive, giving more than a 100-fold reduction in activity when large residues replace glycine. Gly-20 in PlnJ was replaced, the activity was reduced less than 10-fold.

    • Helical Wheel Diagram

    • DRAMP00128 helical wheel diagram
  • 2KHF-> 

    • Predicted Structure

    • There is no predicted structure for DRAMP00128.

Physicochemical Information

    • Formula

    • C133H198N42O34

    • Absent Amino Acids

    • CHMPQTV

    • Common Amino Acids

    • GR

    • Mass

    • 2929.3

    • PI

    • 10.93

    • Basic Residues

    • 6

    • Acidic Residues

    • 2

    • Hydrophobic Residues

    • 9

    • Net Charge

    • +4

    • Boman Index

    • -70.24

    • Hydrophobicity

    • -0.932

    • Aliphatic Index

    • 43.2

    • Half Life

      • Mammalian:30 hour
      • Yeast:>20 hour
      • E.coli:>10 hour

    • Extinction Coefficient Cystines

    • 12490

    • Absorbance 280nm

    • 520.42

    • Polar Residues

    • 8

DRAMP00128

DRAMP00128 chydropathy plot

Comments Information

    • Function

    • A 1

Literature Information

  • ·Literature 1

    • Title

    • Antagonistic activity of Lactobacillus plantarum C11: two new two-peptide bacteriocins, plantaricins EF and JK, and the induction factor plantaricin A.

    • Reference

    • Appl Environ Microbiol. 1998 Jun;64(6):2269-2272.

    • Author

    • Anderssen EL, Diep DB, Nes IF, Eijsink VG, Nissen-Meyer J.
  • ·Literature 2

    • Title

    • Three-dimensional structure of the two-peptide bacteriocin plantaricin JK.

    • Reference

    • Peptides. 2009 Sep;30(9):1613-1621.

    • Author

    • Rogne P, Haugen C, Fimland G, Nissen-Meyer J, Kristiansen PE.