General Information

    • DRAMP ID

    • DRAMP00437

    • Peptide Name

    • Pisum sativum defensin 2 (Psd2; Plant defensin)

    • Source

    • Pisum sativum (Garden pea)

    • Family

    • Belongs to the DEFL family

    • Gene

    • Not found

    • Sequence

    • KTCENLSGTFKGPCIPDGNCNKHCRNNEHLLSGRCRDDFRCWCTNRC

    • Sequence Length

    • 47

    • Protein Existence

    • Protein level

Activity Information

    • Biological Activity

    • Antimicrobial, Antifungal

    • Target Organism

      • PD: Aspergillus niger (MIC=10.2 µg/ml), Aspergillus versicolor (MIC=0.34 µg/ml), Fusarium moniliforme (MIC=10.0 µg/ml), Fusarium oxysporum (MIC>100 µg/ml), Fusarium solani (MIC=8.5 µg/ml), Neurospora crassa (MIC<0.5 µg/ml), Saccharomyces cerevisae (MIC>100 µg/ml), Trichophyton mentagrophytes (MIC<100 µg/ml).
      • PD+: Aspergillus niger (MIC=7.8 µg/ml), Aspergillus versicolor (MIC=5.9 µg/ml), Fusarium moniliforme (MIC=25 µg/ml), Fusarium oxysporum (MIC>100 µg/ml), Fusarium solani (MIC=5.0 µg/ml).
      • NOTE: PD+ = potato dextrose medium supplemented with 1 mM CaCl2.

    • Hemolytic Activity

      • No hemolysis information or data found in the reference(s) presented in this entry

    • Cytotoxicity

    • No cytotoxicity information found in the reference(s) presented

    • Binding Target

    • Not found

Structure Information

    • Linear/Cyclic

    • Cyclic

    • N-terminal Modification

    • Free

    • C-terminal Modification

    • Cyclization of a C-terminal Cys residue (forming a disulfide bond)

    • Nonterminal Modifications and Unusual Amino Acids

    • There are four disulfide bonds between Cys3 and Cys47, Cys14 and Cys35, Cys20 and Cys41, Cys24 and Cys43.

    • Stereochemistry

    • L

    • Structure

    • Bridge

    • Structure Description

    • Not found

    • Helical Wheel Diagram

    • DRAMP00437 helical wheel diagram

    • PDB ID

    • None

    • Predicted Structure

    • There is no predicted structure for DRAMP00437.

Physicochemical Information

    • Formula

    • C219H346N76O69S8

    • Absent Amino Acids

    • AMQVY

    • Common Amino Acids

    • C

    • Mass

    • 5404.1

    • PI

    • 8.52

    • Basic Residues

    • 10

    • Acidic Residues

    • 5

    • Hydrophobic Residues

    • 7

    • Net Charge

    • +5

    • Boman Index

    • -152.73

    • Hydrophobicity

    • -1

    • Aliphatic Index

    • 33.19

    • Half Life

      • Mammalian:1.3 hour
      • Yeast:3 min
      • E.coli:2 min

    • Extinction Coefficient Cystines

    • 6000

    • Absorbance 280nm

    • 130.43

    • Polar Residues

    • 23

DRAMP00437

DRAMP00437 chydropathy plot

Comments Information

    • PTM

    • Contains four disulfide bonds 3-47; 14-35; 20-41; 24-43.

Literature Information

  • ·Literature 1

    • Title

    • Characterization of two novel defense peptides from pea (Pisum sativum) seeds.

    • Reference

    • Arch Biochem Biophys. 2000 Jun 15;378(2):278-286.

    • Author

    • Almeida M.S, Cabral K.M, Zingali R.B, Kurtenbach E.